[Effect of reduced fat content of the diet of the rat and a single fat load on the lipopexic function of the lungs and the development of silicosis].

A diet with low fat content did not influence the lipid level in the lungs and fibrinogenesis values in the development of silicosis in rats, due to the intensification of the lung lipopexic function, that was revealed in the prefibrosis period, using single fat load. A direct correlation of the lung lipopexic function degree in the prefibrosis period with the degree of pneumophibrosis developing afterwards was noted. The optimum time for the study of the rat lung lipopexic function was specified (1.

[Problem of clinico-pathogenetic analysis and correction of behavior disorders in adolescents].

On the basis of integrated clinical, dynamic, and neurophysiological examinations of 542 children and adolescents with behaviour deviations a high (54.05%) percentage of psychic anomalies (primarily, characterological ones) was discovered. The structure of the delinquent behaviour was found to be the most complicated in children and adolescents taught at special schools for juvenile offenders.

[Expression of plasmid R6K in a cell-free coupled transcription-translation system].

The modified method for obtaining E. coli extract S 30 for the coupled transcription--translation system is described. The extract treated with immobilized DNAase and micrococcal nuclease had the minimal endogenous protein synthesis and high activity in respect to exogenous DNA matrices.

Response of a phagocyte cell system to products of macrophage breakdown as a probable mechanism of alveolar phagocytosis adaptation to deposition of particles of different cytotoxicity.

The adaptation of the alveolar phagocytosis response to the quantitative and qualitative features of dust deposited during inhalation consists not only in enhanced recruitment of alveolar macrophages (AM), but also in adding a more or less pronounced neutrophil leukocyte (NL) recruitment as an auxiliary participant of particle clearance. The NL contribution to clearance is especially typical for response to cytotoxic particles (quartz, in particular). An important feature of the adaptation considered is the limitation of the number of AM and NL recruited when an efficient clearance can be achieved by a lesser number of cells due to increased AM reistance to the damaging actin of phagocytized particles.

[Transforming activity of plasmid R6K DNA on Serratia marcescens strain 20-10. The behavior of the plasmid in the transformants].

The authors described transformation of S. marcescens, strain 20-10, of the isolated R6K plasmide DNA. As demonstrated by centrifugation in cesium chloride gradient and electrophoresis in agarose, the plasmide was present in the transformants in the form identical to R6K in E.

[Characteristics of the deletion mutant of plasmid R6K].

Deletion plasmide R6Kdelta with the mol wt of 17.2.10(6) dalton isolated from the E.

[Effect of different edible fats on the lipid metabolism of rats with experimental silicosis].

With a long-term (1 and 4 months) introduction of an additional amount of edible fats (beef, hog fats, butter, sunflower seed oil) to intact and intratracheally quartz-dust laden sexually mature male rats an organ-specific reaction to the supply of fat, and in intact rats, also some peculiarities of the reaction depending upon the kind of the introduced fats, were discovered. An additional administration of fats to rats with developing silicosis tended to increase accumulation of lipids in the lungs and led to a higher content of oxyproline in the lungs and liver, which bears proof to an adverse effect produced by the introduced fats on the lipids metabolism and the processes of silicotic fibrogenesis.

[Entobacterial transformation by R6K plasmid DNA].

Preparations of DNA of R6K plasmide obtained by various methods on the basis of the "clarified" lysate: by gel filtration on sepharose 4B, centrifugation in cesium chloride with ethidium bromide gradient, were analysed in the E. coli C600. S.

[Effect of fat loading on the development of silicosis].

Rats were administered with quartz dust intratracheally or subjected to chronic inhalation of the dust. Peroral overloading of rats with fat was shown to increase an accumulation of all lipid fractions in lungs and intensified silicotic fibrinogenesis. Similar alterations in lipid metabolism of lungs were observed in control animals maintained without the dust treatment at the same fatty diet; but these patterns were less distinct and were not accompanied by an increased collagene formation.