Prikl Biokhim Mikrobiol
May 1998
A method for immobilizing pancreation on carboxymethylcellulose (CMC) is proposed. The optimum reaction conditions were determined: CMC, 1 x 10(-4) M; 1-ethyl 3-(dimethylaminopropyl)-carbodiimide, 2 x 10(-4) M; incubation time, 18 h at 4 degrees C. They allow obtaining a conjugate of the enzyme with the polymer displaying 85% of the protease and 92% of the esterase activity.
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