The importance of targeted next-generation sequencing based genomic profiling in the diagnosis of childhood acute myeloid leukemia: a single center experience.

Background: The management of pediatric acute myeloid leukemia (AML) is based on the prognostic risk classification of initial leukemia. Targeted next-generation sequencing (NGS) is a reliable method used to identify recurrently mutated genes of pediatric AML and associated prognosis.

Methods: In this study, we retrospectively evaluated the prognostic, and therapeutic utility of a targeted NGS panel covering twenty-five genes, in 21 children with de novo and 8 with relapsed or secondary AML.

Exploring in vitro efficacy of rCHAPk with antibiotic combinations, and promising findings of its therapeutic potential for clinical-originated MRSA wound infection.

The increasing threat of antimicrobial-resistant bacteria, particularly Staphylococcus aureus, which rapidly develops multidrug resistance and commonly colonizes wound surfaces, demands innovative strategies. Phage-encoded endolysins offer a dual-purpose approach as topical therapies for infectious skin wounds and synergistic agents to reduce high-dose antibiotic dependence. This study explores recombinant CHAPk (rCHAPk), efficiently synthesized within 3 h, displaying broad-spectrum antibacterial activity against 10 Gram-positive strains, including resistant variants, with rapid bactericidal kinetics.

Status of IKZF1 Deletions in Diagnose and Relapsed Pediatric B-ALL Patients.

IKZF1 deletions (ΔIKZF1) are common in precursor B-cell acute lymphoblastic leukemia (B-ALL) and are assumed to have a prognostic impact. We aimed to determine the prognostic implications of ΔIKZF1 and CRLF2 overexpression in pediatric B-ALL. Furthermore, we sought to compare the multiplex polymerase chain reaction (PCR) assay with standard multiplex ligand-dependent probe amplification (MLPA) methods to ascertain IKZF1 status in a clinical context.

Haematopoietic stem cell transplantation in children with inborn errors of immunity: A single centre experience.

This study retrospectively analyzed the outcomes of 61 pediatric patients with inborn errors of immunity (IEI) who underwent hematopoietic stem cell transplantation (HSCT) between 2011 and 2023. Patients were categorized into primary immunodeficiency disorders (PIDD), primary immune dysregulation disorders (PIRD), and congenital defects of phagocyte number or function (CDP). Median ages at diagnosis and HSCT were 9 and 30 months, respectively.

Optimization of the Filament Winding Process for Glass Fiber-Reinforced PPS and PP Composites Using Box-Behnken Design.

Filament winding is a widely used out-of-autoclave manufacturing technique for producing continuous fiber-reinforced thermoplastic composites. This study focuses on optimizing key filament winding process parameters, including heater temperature, roller pressure, and winding speed, to produce thermoplastic composites. Using Box-Behnken response surface methodology (RSM), the study investigates the effects of these parameters on the compressive load of glass fiber-reinforced polypropylene (GF/PP) and polyphenylene sulfide (GF/PPS) composite cylinders.

The frequency and clinical features of posttransplant pneumonia in solid-organ transplantation recipients: A transplant center experience.

Introduction: In solid-organ transplant (SOT) recipients, while survival rates have improved with immunosuppressive therapies, the risk of opportunistic infections has also increased. This study aimed to evaluate the frequency of pneumonia, identify microbiological factors, investigate diagnostic methods, and analyse prognosis.

Materials And Methods: A retrospective study was conducted to identify adult SOT recipients referred to the pulmonary diseases department with a preliminary pneumonia diagnosis between 2011 and 2019.

The Effect of Preoperative Tele-nursing Counseling on Anxiety and Patient Satisfaction in Day Surgery: A Randomized Controlled Trial.

Purpose: This study aimed to investigate the effect of preoperative tele-nursing counseling on anxiety and patient satisfaction in day surgery.

Design: Randomized controlled study design was employed.

Methods: The study was conducted in a city hospital and a university hospital in Istanbul between July 2022 and May 2023 with patients who met the study criteria.

Impact of weight change on kidney transplantation outcomes: A systematic review and meta-analysis.

Background And Aim: Kidney transplant recipients frequently experience a wide range of metabolic complications, including weight changes, which significantly impact patient outcomes and graft function, yet the relationship between weight gain and transplant outcomes remains poorly understood. This systematic review and meta-analysis aimed to synthesise existing evidence on the influence of weight gain on patient and graft outcomes following kidney transplantation to enhance clinical practice and optimise post-transplant care strategies.

Materials And Methods: A literature search was conducted across databases such as PubMed and Scopus for peer-reviewed studies published up to 8 August 2024.

Prospective comparison of two different surgical technique in the treatment of deep osteochondral lesions of the talus: mosaicplasty versus all-arthroscopic technique with scaffold and autograft bone taken from the tibial plafond.

Introduction: There are various surgical techniques applied for the treatment of osteochondral lesions of the talus (OLT). The mosaicplasty technique is one of the most commonly used methods for deep lesions. The all-arthroscopic technique with scaffold and autograft bone taken from the tibial plafond is a novel method as an alternative to the mosaicplasty.

Analysis of bioactive compounds of hen egg components at the first half of incubation.

A comparative analysis of mass-volume characteristics of structural and morphological components of hen eggs before incubation and on the fifth (HH25-HH27) and 10th (HH36) days of incubation was carried out. During incubation, egg weight decreased by 9.25% (10 days), mainly due to a decrease in albumen weight (35.

The Effect of Iron Deficiency Anemia on Emergency Department Admission in Asthmatic Children.

Background: Iron deficiency (ID) is the most common nutritional deficiency and cause of anemia in childhood. Iron deficiency anemia (IDA) is a common public health problem in Turkey as in the rest of the World. Asthma is a chronic inflammatory disease of the respiratory tract, affecting approximately 10% of the population in developing countries.

Inhibitor development upon switching from plasma-derived to recombinant factor VIII in previously untreated patients with severe hemophilia A: the PUP-SWITCH study.

Background: The SIPPET randomized clinical trial showed that in previously untreated patients (PUPs) with severe hemophilia A, treatment with plasma-derived factor (F)VIII (pdFVIII) within the first 50 exposure days (EDs) was associated with a lower cumulative incidence of inhibitors than with recombinant FVIII (rFVIII). Switching to rFVIII beyond 50 EDs with pdFVIII is a treatment often implemented by many centers. The question is whether or not this switch may induce a risk of inhibitor development.

Functional knee bracing provides protection to the anterior cruciate ligament in response to rotatory loads.

Background: The aim of the study was to quantify the effect of functional knee bracing on native knee kinematics and the in-situ force in the ACL in response to external loading.

Methods: A robotic testing system was used to apply three external loads from full extension to 60° of flexion to eight fresh frozen human cadaveric knees: 1) a 134 N anterior load, 2) a combined 5-Nm internal rotation +5-Nm valgus torque, and 3) a combined 5-Nm external rotation +5-Nm valgus torque. For native and braced states, kinematics were recorded and the in-situ force in the ACL was determined.

Two new species of mites in the family Pachylaelapidae from Türkiye (Acari: Mesostigmata).

This study reviews some species of mites in the family Pachylaelapidae from Türkiye. Onchodellus marginatus sp. nov.

Isolation, genomic characterization and biotechnological evaluation of lactobacilli strains from chicken gastrointestinal tract.

During a study investigating possible probiotics from chicken gut microbiota, strains C1-4 and C2-3 were isolated and identified as members of the genus Ligilactobacillus. The strains formed a well-supported cluster with Ligilactobacillus salivarius and Ligilactobacillus hayatikensis in phylogenetic trees. Their genomes, sized 1.

Recurrent eosinophilia with a novel homozygous ARPC1B mutation.

Cytoskeletal network dysregulation is a pivotal determinant in various immunodeficiencies and autoinflammatory conditions. This report reviews the significance of actin remodeling in disease pathogenesis, focusing on the Arp2/3 complex and its regulatory subunit actin related protein 2/3 complex subunit 1B (ARPC1B). A spectrum of cellular dysfunctions associated with ARPC1B deficiency, impacting diverse immune cell types, is elucidated.

Understanding Risk Factors for Cytomegalovirus Infection in Pediatric Hematopoietic Stem Cell Transplantation: Insights From a 10-Year Study Comparing Conditioning Regimens.

Hematopoietic stem cell transplantation is a curative therapy for pediatric patients with malignancies and blood or immune system disorders. However, recipients are at risk of opportunistic infections, including cytomegalovirus infection, due to immunosuppression from conditioning regimens. This retrospective study analyzed 439 pediatric hematopoietic stem cell transplantation patients to identify risk factors for cytomegalovirus infection and assess the impact of conditioning regimens.

Molecular and In Silico Analysis of the Gene in Individuals with High Risk of Cancer Predisposition from Türkiye.

() is a tumor suppressor gene involved in DNA repair and cell cycle regulation. Pathogenic or likely pathogenic (P/LP) variants in are associated with increased cancer risk. Conversely, recent large cohort studies have identified certain variants that, despite being classified as P/LP by in silico analysis, are considered low risk.

Retinal Vascular Changes in a Case of Straatsma Syndrome.

A 34-year-old asymptomatic woman with a history of amblyopia in her right eye presented for a routine examination. Fundus examination revealed broad myelinated nerve fibers along with telangiectatic vessels. Wide-field fluorescein angiography revealed no evidence of ischemia or active retinal neovascularization.

Post-transplant IgA nephropathy: a rapidly evolving field of kidney transplant medicine.

IgA nephropathy is the commonest pattern of primary glomerular disease in the world, with high rates of progression to kidney failure. As IgA nephropathy commonly causes kidney failure at a young age, kidney transplantation is commonly used to treat kidney failure. However, high rates of recurrent disease in the allograft remain a common management challenge.

Spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography of mice.

Optoacoustic (OA) tomography is a powerful noninvasive preclinical imaging tool enabling high resolution whole-body visualization of biodistribution and dynamics of molecular agents. The technique yet lacks endogenous soft-tissue contrast, which often hampers anatomical navigation. Herein, we devise spiral volumetric optoacoustic and ultrasound (SVOPUS) tomography for concurrent OA and pulse-echo ultrasound (US) imaging of whole mice.

The detection of distomolar teeth on panoramic radiographs using different artificial intelligence models.

Purposes: One notable anomaly, presence of distomolars, arises beyond the typical sequence of the human dental system. In this study, convolutional neural networks (CNNs) based machine learning methods were employed to classify distomolar tooth existence using panoramic radiography (PR).

Methods: PRs dataset, composed of 117 subjects with distomolar teeth and 146 subjects without distomolar teeth, was constructed.

Management of persistent exudative retinal detachment: exploring etiology and surgical outcomes.

Background: This report aims to present the causes, clinical features, and surgical outcomes of persistent exudative retinal detachment (ERD) in a single tertiary referral center experience.

Methods: We retrospectively analyzed 48 patients who underwent vitreoretinal intervention for persistent ERD between 2017 and 2024. The study assessed patient demographics, underlying causes of ERD, the success rate of surgical interventions, and postoperative visual outcomes.