Polyhydroxyphenylvalerate/polycaprolactone nanofibers improve the life-span and mechanoresponse of human IPSC-derived cortical neuronal cells.

The physico-chemical characteristics of the extracellular matrix (ECM) cause mechanical cues that could elicit responses in the survival rate of cortical neuronal cells. Efficient neurite outgrowth in vitro, is critical for successful cultivation of cortical neuronal cells and the potential for attempts at regeneration of the central nervous system (CNS) in vivo. Relatively soft and hydrophilic, microbially synthesized aromatic polyester, polyhydroxyphenylvalerate (PHPV) was blended 50:50 with the stiff and hydrophobic polycaprolactone (PCL) and electrospun in microfibers for use in a 3D (CellCrown™) configuration and in a 2D coverslip coated configuration.

Low-Temperature Synthesis of Magnetic Carbonaceous Materials Coated with Nanosilica for Rapid Adsorption of Methylene Blue.

This work reports the synthesis of nanosilica-coated magnetic carbonaceous adsorbents (MCA@SiO) using low-temperature hydrothermal carbonization technique (HCT) and the feasibility to utilize it for methylene blue (MB) adsorption. Initially, a carbon precursor (CP) was synthesized from corn starch under saline conditions at 453 K via HCT followed by the magnetization of CP again via HCT at 453 K. Subsequently, MCA was coated with silica nanoparticles.

Rapid, Phase-free Detection of Long Identity-by-Descent Segments Enables Effective Relationship Classification.

Identity-by-descent (IBD) segments are a useful tool for applications ranging from demographic inference to relationship classification, but most detection methods rely on phasing information and therefore require substantial computation time. As genetic datasets grow, methods for inferring IBD segments that scale well will be critical. We developed IBIS, an IBD detector that locates long regions of allele sharing between unphased individuals, and benchmarked it with Refined IBD, GERMLINE, and TRUFFLE on 3,000 simulated individuals.

Photoelectric performance evaluation of DSSCs using the dye extracted from different color petals of Leucanthemum vulgare flowers as novel sensitizers.

In this work, the natural flower extracted dyes containing luteolin were prepared using three different specimens from daisy flowers family (Leucanthemum vulgare), namely yellow daisy, purple daisy and wine daisy, according to the color of its petals. Moreover, DSSCs were fabricated using nanosized titanium dioxide (TiO) as an anode; for the photocathodes, two different specimens were used: i) graphite electrode and ii) platinum electrode. To recognize the light absorption behavior, the existence of anchoring groups and coloring components of the extracted dyes were determined using absorption spectroscopy.

Leveraging Technology for Nation-Wide Training of Healthcare Professionals in Cancer Screening in India: a Methods Article.

The Indian Council of Medical Research-National Institute of Cancer Prevention and Research (ICMR-NICPR) has been conducting online cancer screening training certificate courses since 2017. Thereafter, multiple cohorts have been trained successfully in cancer screening using the Extensions for Community Healthcare Outcomes (ECHO) platform. A 14-week course was designed for various cadres of healthcare professionals (HCP), through which they were trained in cancer screening and their roles and responsibilities in implementing the population-based cancer screening, as per the operational framework released by the Ministry of Health and Family Welfare.

Locate, Size, and Count: Accurately Resolving People in Dense Crowds via Detection.

We introduce a detection framework for dense crowd counting and eliminate the need for the prevalent density regression paradigm. Typical counting models predict crowd density for an image as opposed to detecting every person. These regression methods, in general, fail to localize persons accurate enough for most applications other than counting.

Alternatives to Biological Skin in Permeation Studies: Current Trends and Possibilities.

The transdermal route of drugs has received increased attention in recent years due to numerous advantages over the oral and injectable routes, such as avoidance of the hepatic metabolism, protection of drugs from the gastrointestinal tract, sustained drug delivery, and good patient compliance. The assessment of ex vivo permeation during the pharmaceutical development process helps in understanding the product quality and performance of a transdermal delivery system. Generally, excised human skin relevant to the application site or animal skin is recommended for ex vivo permeation studies.

Flavonoids as Multi-Target Compounds: A Special Emphasis on their Potential as Chemo-adjuvants in Cancer Therapy.

Flavonoids are low molecular weight, polyphenolic phytochemicals, obtained from secondary metabolism of various plant compounds. They have a spectrum of pharmacological efficacies, including potential anticancer efficacy. Natural flavonoids are present in fruits, vegetables, grains, bark, roots, stems, flowers, tea and wine.

Androgen Receptor Expression in Hypospadias.

Introduction: The exact mechanism behind the development of hypospadias is unclear. Research studies on androgen receptor (AR) expression are controversial with results stating all possible outcomes - AR elevated, similar, or reduced when compared to normal.

Aims: The aim is to study the AR expression and hormone levels in hypospadias patients and compare them with children having normal genitalia.

Complications of Percutaneous Drainage in Step-Up Approach for Management of Pancreatic Necrosis: Experience of 10 Years from a Tertiary Care Center.

Introduction: Percutaneous catheter drainage (PCD) as initial intervention in necrotizing pancreatitis has led to improved outcomes and obviated need for surgery in a significant proportion. However, there can be difficulty in accessing deep-seated necrotic collections by percutaneous catheter and complications are likely.

Methods: The present study involves a retrospective analysis of a prospectively maintained database of patients with necrotizing pancreatitis managed by a step-up approach.

Utilization of gum xanthan based superporous hydrogels for the effective removal of methyl violet from aqueous solution.

In this research work the superporous hydrogels (SPHs) of acrylic acid (AA) and acrylamide (AM) with gum xanthan (GX) were synthesized using glass blowing and foaming technique and tested the feasibility of synthesized SPHs to remove removal of methyl violet dye (MV) by the process of adsorption from aqueous solution. Swelling properties of synthesized SPHs with different concentrations of GX were studied in deionized water and the swelling kinetics followed first-order model. The adsorption of MV using SPHs was highly influenced by the solution pH.

Stability-indicating HPLC method for acyclovir and lidocaine in topical formulations.

A simple, rapid and accurate stability-indicating HPLC assay was developed for the determination of acyclovir and lidocaine in topical formulations. Chromatographic separation of acyclovir and lidocaine was achieved using a reversed-phase C column and a gradient mobile phase (20 mm ammonium acetate pH 3.5 in water and acetonitrile).

Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines.

Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc).

A new species of Gomphidia Selys, 1854 (Insecta: Odonata: Anisoptera: Gomphidae) from the Western Ghats of India.

A new species of Gomphidia Selys, 1854 is described from southern Western Ghats, India based on a male specimen. The new species is very distinct from all the known species of Gomphidia from Indian subcontinent. Revised keys to the males and known females of Gomphidia of India and Sri Lanka are provided.

A new record of the family Isonychiidae (Insecta: Ephemeroptera) from the Western Ghats, India with a description of new species.

A new species belonging to the subgenus Isonychia, of the genus Isonychia Eaton, 1871, is described based on larvae and imagoes collected from Moyar River, Nilgiri District, Tamil Nadu, India. The imagoes of I. moyarensis n.

Predrainage and Postdrainage Prognostic Nomograms to Predict Outcome of Percutaneous Drainage for Infected Pancreatic and Peripancreatic Necrotic Collections.

Objectives: This study aimed to identify factors affecting outcome of percutaneous catheter drainage (PCD) in management of infected pancreatic necrosis treated with step-up approach.

Methods: This was a single-center retrospective cohort study that included patients with infected necrosis undergoing PCD as initial intervention. Patients who did not respond underwent necrosectomy.

Serotonin is essential for eye regeneration in planaria Schmidtea mediterranea.

Planaria is an ideal system to study factors involved in regeneration and tissue homeostasis. Little is known about the role of metabolites and small molecules in stem cell maintenance and lineage specification in planarians. Using liquid chromatography and mass spectrometry (LC-MS)-based quantitative metabolomics, we determined the relative levels of metabolites in stem cells, progenitors, and differentiated cells of the planarian Schmidtea mediterranea.

How to Save Half a Million Dollars: An Antimicrobial Stewardship Program in a Tertiary Care Center.

We started a quality improvement project (QIP) with the aim of implementing an antimicrobial stewardship program (AMSP) to optimize antimicrobial use. We implemented this QIP in our tertiary care center with baseline data from July 1, 2017, to December 31, 2017 (pre-AMSP period), and the project period between January 1, 2018, and June 30, 2018. It covered every inpatient with a positive microbiological culture and patients who were initiated on a pre-selected list of 16 restricted antimicrobials.

Evaluation of nonlinear optical properties from molecular descriptors of benzimidazole metal complexes by principal component analysis.

In the present work, semiempirical quantum chemical method (PM6) has been used for the simulation of molecular descriptors of first row transition metal ions incorporated benzimidazole metal complexes. Metal complexes with and without substituents are considered for the analysis. In both the cases, molecules show distinct properties with respect to the molecular descriptors.

Comparison of different pathological markers in predicting pyeloplasty outcomes in children.

Purpose: To compare the efficacy of pathological markers like Interstitial cells of Cajal (ICC), neurons and Collagen to Muscle ratio (CM ratio), in predicting pyeloplasty outcomes.

Methods: Histological sections from 31 patients with UPJO were analyzed for ICC & neurons on immuno-histochemistry and CM ratio on Masson's trichrome staining. Post-operative outcomes were analyzed at 1-year follow up; expressed as excellent, moderate or mild improvement, static and deterioration based on the three factors: ultrasound grade, differential renal function and renogram drainage pattern.

Causing Brain Abscess.

Nocardial brain abscess is a rare central nervous system infection with high morbidity and mortality. Most of the human infections, i.e.

Elevation of gene expression of calcineurin, calmodulin and calsyntenin in oxidative stress induced PC12 cells.

In normal physiological conditions, reactive oxygen and nitrogen species are used as important signaling molecules in the cell. However, in excess it causes the disruption of cell resulting in their death. Oxidative stress causes influx in intracellular calcium levels leading to higher concentrations of calcium in the cell.

Conversion of waste cooking oil into medium chain polyhydroxyalkanoates in a high cell density fermentation.

Biodegradable and biocompatible polymers polyhydroxyalkanoates (PHAs) have a wide range of applications from packaging to medical. For the production of PHA at scale it is necessary to develop a high productivity bioprocess based on the use of a cheap substrate. The objective of the current study was to develop a high cell density bioreactor-based process for the production of medium chain length polyhydroxyalkanoate (mclPHA) with waste cooking oil as the sole carbon and energy source.

Role of Ceramides in Drug Delivery.

Ceramides belong to the sphingolipid group of lipids, which serve as both intracellular and intercellular messengers and as regulatory molecules that play essential roles in signal transduction, inflammation, angiogenesis, and metabolic disorders such as diabetes, neurodegenerative diseases, and cancer cell degeneration. Ceramides also play an important structural role in cell membranes by increasing their rigidity, creating micro-domains (rafts and caveolae), and altering membrane permeability; all these events are involved in the cell signaling. Ceramides constitute approximately half of the lipid composition in the human skin contributing to barrier function as well as epidermal signaling as they affect both proliferation and apoptosis of keratinocytes.