Proteomics of human aqueous humor.

The aqueous humor is a colorless, transparent fluid that fills the anterior chamber of the eye. It plays an important role in maintaining the intraocular pressure and providing nourishment to the lens and cornea. The constitution of the aqueous humor is controlled by the blood-aqueous barrier.

Activation of diverse signalling pathways by oncogenic PIK3CA mutations.

The PIK3CA gene is frequently mutated in human cancers. Here we carry out a SILAC-based quantitative phosphoproteomic analysis using isogenic knockin cell lines containing 'driver' oncogenic mutations of PIK3CA to dissect the signalling mechanisms responsible for oncogenic phenotypes induced by mutant PIK3CA. From 8,075 unique phosphopeptides identified, we observe that aberrant activation of PI3K pathway leads to increased phosphorylation of a surprisingly wide variety of kinases and downstream signalling networks.

Serum ferroxidase albumin ratio as a marken in pulmonary tuberculosis.

Serum ferroxidase and albumin levels were determined in 98 patients of tubercuiosis, of whom 49 were freshly diagnosed, sputum positive (group-I) & 49 were completely treated patients (group-II). Forty nine age and sex matched healthy individuals were taken as controls. Mean±SD of serum ferroxidase and albumin levels in controls, group-I and group-II was found to be 864.

A kinetic method amenable to automation for ceruloplasmin estimation with inexpensive and stable reagents.

Objectives: To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase.

Design And Methods: Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric.

Results: Mean ferroxidase activity in healthy adults, Wilson's disease and pregnancy was 787.