J Autom Methods Manag Chem
June 2010
The new selective access analysis system BM/Hitachi 917 was evaluated in an international multicentre study, mainly according to the ECCLS protocol for the evaluation of analysers in clinical chemistry. Forty-three different analytes, covering 56 different methods--enzymes, substrates, electrolytes, specific proteins, drugs and urine applications--were tested in seven European clinical chemistry laboratories. Additionally, the practicability of the BM/ Hitachi 917 was tested according to a standardized questionnaire.
View Article and Find Full Text PDFThe analytical performance of Elecsys 2010 has been assessed in a multicentre evaluation, which involved twelve laboratories from eight countries worldwide. Eleven alanytes (TSH, T4, FT4, T3, FT3, T-up, TNT, CK-MB, HCG, CEA and PSA) were tested using a protocol related to the ECCLS guidelines and the Standard Operating Procedures of the manufacturer. The evaluation was supported by a "Computer Aided Evaluation" (CAEv) program system and telecommunications.
View Article and Find Full Text PDFThe analytical performance and practicability of the Boehringer Mannheim (BM)/Hitachi 911 analysis system have been assessed in a multicentre evaluation, which involved six laboratories from European countries. Analytes commonly used in classical clinical chemistry were tested in a core programme, which mainly followed the ECCLS guidelines. In addition, a satellite programme covered other analytes, such as proteins, drugs and urine analytes.
View Article and Find Full Text PDF187 patients were checked up over 4 years by the secretin-ceruletide test. Independently of the test results they were assigned to various disease groups on the basis of clinical assessment. 131 subjects were divided in a pilot investigation into: subjects with a healthy pancreas (n = 55); subjects with chronic pancreatitis (n = 50); subjects whose pancreatic condition could not be classified clearly (n = 26).
View Article and Find Full Text PDFThe evaluation of new reagents and instruments in clinical chemistry leads to complex studies with large volumes of data, which are difficult to handle. This paper presents the design and development of a program that supports an evaluator in the definition of a study, the generation of data structures, communication with the instrument (analyser), online and offline data capture and in the processing of the results. The program is called CAEv, and it runs on a standard PC under MS-DOS.
View Article and Find Full Text PDFThe biometrical treatment of laboratory data may require the estimation of a regression line for the transformation of one set of measurements to another. The regression procedure introduced in part I (1) of our work does not always yield unbiased results in such situations, since its estimators are not scale invariant. In part III we present the parameter estimation of a general regression equation which is scale invariant and retains all properties of the method comparison procedure, in particular its robustness.
View Article and Find Full Text PDFJ Clin Chem Clin Biochem
December 1987
The selective multitest Boehringer Mannheim/Hitachi 704 analysis system was examined according to the ECCLS guidelines in a multicentre evaluation involving four laboratories. Ten routine parameters, covering most of the application settings of the instrument, were measured in the respective laboratory at temperatures 25, 30 or 37 degrees C. The trial lasted four months and gave more than 40,000 data.
View Article and Find Full Text PDFDiabetes Care
September 1983
Diabur-Test 5000, a new test strip for urinary glucose, permits the accurate measurement of up to 5% glucose concentrations in urine. In a survey carried out at 12 diabetes centers in Europe, the new test strip was tested in laboratory trials and routine self-monitoring. There was good agreement between the test strip and the quantitative glucose determination on 4105 urine samples in the laboratory trials.
View Article and Find Full Text PDFTwo commercial systems for testing the antimicrobial susceptibility of bacteria were evaluated in a collaborative study at two sites using 306 freshly isolated clinical strains. Gram-negative bacteria were tested with the Micur RST 02 and gram-positive bacteria with the Micur RST 03, systems for MIC titration. For categorization of bacterial susceptibility into sensitive, intermediate or resistant, the Micur ST 02 and 03 were used for gram-negative and gram-positive bacteria respectively.
View Article and Find Full Text PDFJ Clin Chem Clin Biochem
December 1981
From the study described in part 1 of this series (Passing, H. et al. (1981), this j.
View Article and Find Full Text PDFA new kinetic assay for antithrombin III (heparin-cofactor) in plasma was used to determine reference values. Ranges of 10--15 IU/ml at 25 degrees C and 20--29 IU/ml at 37 degrees C were found for a reference population of 219 men and 204 women 15--93 years of age. There was no evidence that these values vary with age or sex, nor was there any difference between pre- and postprandial antithrombin activities, or between smokers' and non-smokers' values.
View Article and Find Full Text PDFDtsch Med Wochenschr
February 1981
Development of a new specific test for estimation of pancreatic lipase activity (EC 3.1.1.
View Article and Find Full Text PDFReference values for 327 children aged between 1 day and 15 years are given for the revised standard method for the determination of serum creatine kinase (CK) activity by activation with N-acetylcysteine (NAC). No sex-dependence could be observed, but CK activity was found to vary markedly with age. The following values are suggested as upper limits of the reference ranges for measurements at 25 degrees C: neonates: 1-3 days: less than or equal to 370 U/l, 4-10 days: less than or equal to 200 U/l, 11-31 days: less than or equal to 100 U/l; children (2 months-15 years): less than or equal to 90 U/l.
View Article and Find Full Text PDFTests in five laboratories on pooled sera in three concentration ranges demonstrated good reproducibility of urea concentration with the Reflotest-Urea, at a variation coefficient from 2.4% to 5.0%.
View Article and Find Full Text PDFJ Clin Chem Clin Biochem
November 1979
The creatinine concentrations in 337 sera were determined by enzymatic assay and by six different modifications of the Jaffé method. The enzymatic assay showed satisfactory precision and gave the same results as the assay after adsorption on Fuller's earth (Lloyd's reagent). All other methods using picrate gave falsely high values: the relative deviation from the enzymatic method had a median value between +20% and +50% for creatinine concentrations in the range 22-88 mu mol/l, and between +10% and 30% for creatinine concentrations in the range 89-177 mu mol/l.
View Article and Find Full Text PDFJ Clin Chem Clin Biochem
November 1979
Reference values for creatinine in serum were established using a fully enzymatic method, adapted to a discontinuous analyzer. Our reference group included 250 females and 215 males (blood donors, hospital staff and patients) aged 18-70 years. Up to 60 years, creatinine concentration did not depend on age, but there was a significant difference between the creatinine concentrations of women and men.
View Article and Find Full Text PDFWhite-cell count and differential blood count, total protein, lactate dehydrogenase activity (E.C. 1.
View Article and Find Full Text PDFEur J Clin Pharmacol
November 1978
Ple 1053 (Azosemid) is a diuretic which resembles furosemide chemically and in its mode of action. When administered intravenously, Ple 1053 was approximately 5 times more potent on a weight basis than furosemide, its dose-response relationship was closer and the slope was steeper. After oral administration Ple 1053 and furosemide were approximately equal in potency.
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