[Features of treating patients with combined damage to the orbit and eye in the late period in peacetime and war conditions].

Analysing the diagnosis and treatment of 100 patients with combined injuries of the organ of vision and orbital zone of the face provided clinical features of lesions in peace-time or battle accidents. Battle accidents were found to be prognostically worse for visual functions. The use of immunotherapy for inflammatory complications of a traumatic process is demonstrated to be advisable.

[Role of immune complexes in thyroid diseases].

To elucidate the immune complex (thyroglobulin-antibody, Tg-Ab) role in thyroid hormone formation, in vitro iodination of Tg--AB immune complex, bovine intact Tg and that of the patient with euthyroid goiter and partially purified AB to Tg were studied. We compared the amount of iodamine acids (MIT, DIT, T4/mole of Tg) forming during iodination in immune complex with other samples. The results suggest that Tg being in complex with antibodies (up to 30-40 mole of antibodies/mole of Tg) is iodinated forming the enough amount of MIT, decreased amount of DIT and T4.

[Isolation of thyroglobulin from the bovine thyroid free of endogenous proteolytic activity].

Thyroglobulin was isolated from the cattle thyroid gland by chromatography on Sephadex G-200 and Sepharose 4B. It was found homogeneous according to disc electrophoresis (pH 8.6) and analytical ultracentrifugation.

[Membrane activity of a cell-free filtrate of Shigella flexneri 2a].

In the course of metabolism in Shigella Flexneri 2A strain there was noted secretion of a membrane-tropic factor into media. This factor increased the conductivity of bilayer lipid membranes causing formation of ionic channels in them. Minimal conductivity of the channels was about 25 pS in 0.

[Differential spectrophotometry of iodinated proteins and amino acids in heavy water].

Differential absorption spectra of alkaline solutions of monoiodotyrosine and thyroxine with 90% D2O as perturbant were measured and characterized. The obtained spectra are mainly determined by the difference of dielectric constants.

[Improved accuracy of a direct spectrophotometric method of determining iodotyrosines and thyroxine in iodinated proteins].

The accuracy of spectrophotometric quantitation of iodinated amino acid residues in proteins was enhanced 2,6--7,0-fold by appropriate choice of wavelengths.

[The role of thyroglobulin carbohydrates in its hormone-liberating function. Proteolysis of deglycosylated thyroglobulin].

The proteolysis of thyroglobulin was performed after partial cleavage of its carbohydrate moiety. It was demonstrated that enzymatic removal of some thyroglobulin sialic acids results in increasing its resistance to proteolysis. Apparently, sialic acids are essential for maintaining an optimal thyroglobulin conformation for the action of proteases at hormonal biosynthesis from the reserve form.

[Differences in the secondary structure of thyroglobulin under normal conditions and in nodular goiter].

Intact and iodine-treated thyroglobulin from normal thyroid gland and from nodular euthyroid goiter were studied by means of circular dichroism to detect differences in the protein secondary structure. Contents of alpha- and beta-structures in thyroglobulins were differentiated only by several per cents but artificial iodination of the protein increased markedly the difference between thyroglobulins of various origin. Extrapolation of the data to "zero" content of iodine-containing amino acids demonstrated that the initial steps of thyroglobulin synthesis occurring before iodination "in vitro", appears to be also responsible for alterations in the protein secondary structure.

[Condensation of residues of diiodotyrosine with thyroxine near the surface of the thyroglobulin molecule by means of differential spectrophotometry].

Aromatic amino acids including iodotyrosines and thyroxin in intact, extraiodinated and acetylated bovine thyroglobulin were studied by the differential spectrophotometric technique. Modifications (i. e.

[The inherent fluorescence of thyroglobulin].

Properties of tryptophane and tyrosine fluorescence of intact and iodinated thyroglobulin from normal human thyroid and nodular euthyroid goiter were studied. It has been shown that practically all (95%) tryptophane residues in "normal" thyroglobulin are in the inner regions of the globule. In "pathological" thyroglobulin in the regions inaccessible for water there are located only 68% of trypthophanyls.

Differential spectrophotometry of iodoamino acids and iodinated proteins. Aqueous ethylene glycol solutions of iodotyrosines, thyroxine and thyroglobulin.

Solvent perturbation difference spectra and corresponding molar extinction coefficients of solutions of monoiodotyrosine, diiodotyrosine and thyroxine with protonated and deprotonated hydroxyls were measured with 20% ethylene glycol as perturbant. Data obtained were applicable to the development of the method of quantitative estimation of the number of iodotyrosine and thyroxine residues accessible to ethylene glycol in iodinated proteins. This method was used for the determination of iodoamino acids available to ethylene glycol in bovine thyroglobulin (naturally iodinated protein).

[Extinction coefficient of thyroglobulin].

The extinction coefficient at 280 nm of the solution (pH 7,2-7,4) of thyroglobulin from normal thyroid and nodular euthyroid goiter was measured with the values of 0,6-0,7 to 0,9 ml.mg-1.cm-1.

[Synthesis of iodoamino acids during in vitro thyroglobulin iodination in different states of its molecule].

Iodotyrosine and iodothyronine residues are formed in the protein molecule during bovine thyroglobulin iodination in vitro. Dissociation and reassociation of the thyroglobulin molecule have no significant influence on its iodoaminoacid composition. Thyroglobulin iodination in the presence of 8 M urea does not result in thyroxine synthesis despite the increased formation of iodotyrosine residues.

[14C-1-Tyrosine metabolism on rat thyroid gland].

Amino acid composition of incubation medium, thyroid tissue hydrolysate and purified thyreoglobulin (Tr) preparations, isolated after 5 hour incubation with 14C-1-tyrosine in Jey medium is studied by means of electrophoresis and autoradiography. 5 radioactivity stains are detected on radioelectrophoregram of incubation medium, 2 of them corresponding to tyrosine and serine. 2 stains corresponding to diiodotyrosine and triiodotyronine are found in hydrolysate of thyroid tissue.