Canine epilepsy/seizure occurrence in primary care and referral populations: a look into the epidemiology across countries.

Epilepsy is a common neurological condition in dogs. Analysis of primary care populations across countries can provide a more complete understanding of the epidemiology of this condition and provide context for spectrum of care discussions. This narrative literature review was aimed at understanding canine epilepsy/seizure prevalence in primary care populations, and changes in occurrence across geography, culture, and socioeconomic status.

WHO antenatal care policy and prevention of malaria in pregnancy in sub-Saharan Africa.

Background: The WHO 2016 antenatal care (ANC) policy recommends at least eight antenatal contacts during pregnancy. This study assessed ANC8 uptake following policy implementation and explored the relationship between ANC attendance and intermittent preventive treatment in pregnancy (IPTp) coverage in sub-Saharan Africa following the rollout of the World Health Organization (WHO) 2016 ANC policy, specifically, to assess differences in IPTp uptake between women attending eight versus four ANC contacts.

Methods: A secondary analysis of data from 20 sub-Saharan African countries with available Demographic Health and Malaria Indicator surveys from 2018 to 2023 was performed.

Malaria-Related Psychosocial Factors, Past Antenatal Care-Seeking Behaviors, and Future Antenatal Care-Seeking Intentions by Maternal Age in Malawi and Democratic Republic of the Congo.

Young women in sub-Saharan Africa are a group at increased risk for malaria in pregnancy. Early antenatal care (ANC) seeking makes it more likely that women will receive the recommended doses of intermittent preventive treatment of malaria in pregnancy. This study used data from national Malaria Behavior Surveys conducted in Malawi and the Democratic Republic of the Congo (DRC) in 2021 to explore the association between intention to attend ANC in the first trimester for a future pregnancy (early ANC intention) and psychosocial factors among women aged 15-49 years.

Quality of life in Cushing's syndrome.

Measurement of Health-Related Quality of Life (HRQoL) is emerging as an important clinical endpoint which complements diagnostic workup and contributes to place patients at the centre of the decision-making process through the recognition of their needs, concerns, goals and expectations. Chronic excessive cortisol exposure in Cushing's syndrome (CS) causes severe physical and psychological morbidity which invariably affects HRQoL during the active phase of the disease and even after successful treatment. This sustained deterioration of patient's wellbeing is partly related to the persistence of several features associated with prior cortisol excess, including affective disorders, cognitive dysfunctions and negative illness perception.

Assessing Hepatitis B Knowledge Among Immigrant Communities in New York City.

The objective of this study was to assess the knowledge of Hepatitis B virus (HBV) among New York City immigrants. Data were obtained from cohort of 2385 persons at-risk for HBV, who completed a knowledge assessment survey during HBV screening events in 2010-2013. HBV knowledge scores were very low among the tested population, with the majority answering every question incorrectly.

Analysis of oral lesion biopsies identified and evaluated by visual examination, chemiluminescence and toluidine blue.

Conventional visual examination and palpation remains the gold-standard for the identification of oral mucosal lesions. The purpose of this study was to investigate the adjunctive value of a chemiluminescent light source (ViziLite, Zila Pharmaceuticals, Phoenix, Arizona) and application of pharmaceutical grade toluidine blue (TBlue(630), Zila Pharmaceuticals, Phoenix, Arizona) to further assess lesions identified during the conventional oral soft tissue examination. Lesions deemed clinically suspicious by visual examination under incandescent light were further assessed under chemiluminescence and then application of toluidine blue stain.

Cost of care for early- and late-stage oral and pharyngeal cancer in the California Medicaid population.

Background: This study documents the direct medical costs associated with treating oral and pharyngeal squamous cell carcinoma (OSCC) as early- or late-stage disease according to the current standard of care.

Methods: This retrospective analysis of California Medicaid claims data calculated direct payments for patients diagnosed with OSCC. Patients were defined as being treated for early- or late-stage disease based on treatment modality.

The efficacy of oral lumenoscopy (ViziLite) in visualizing oral mucosal lesions.

Early diagnosis of oral mucosal lesions has been advocated as a means of improving outcomes of cancer therapy. Improved visualization of mucosal lesions may aid in diagnosis by guiding tissue sampling or referral. This multicenter study reports the effect of chemiluminescent light (ViziLite) upon visualization of mucosal lesions.

Structural and immunohistological modifications in olfactory bulb of the staggerer mutant mouse.

In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e.

Antibody-coated electrodes for detecting somatic exocytosis of somatostatin-like material in Helix neurones.

Immunoelectrodes have been developed which can be used to detect minute amounts of somatostatin. They were made with electrochemically treated glassy carbon fibres coated with anti-somatostatin antibodies. Calibration and various controls were carried out to ensure that the immunoelectrodes responded specifically to the presence of femtomolar somatostatin.

In vitro effects of methionine-enkephalin, somatostatin and insulin on cultured gonadal cells of the snail Helix aspersa.

Isolated snail gonadal cells were cultured in the presence of synthetic neuropeptides in order to determine the subsequent effect of these substances on gonadal synthetic activities. Gonadal cells were incubated for 24 h in concentrations of methionine-enkephalin, somatostatin and insulin ranging from 10(-4) M to 10(-9) M, in medium 199 supplemented with 6% Ultroser G. Synthesis of DNA and protein by the cultured cells were simultaneously estimated by measuring incorporation of 3H thymidine and 35S methionine.

[Modifications of the olfactory bulb electrocorticogram and trans-glomerular evoked potentials in staggerer mutant mice].

Electrophysiological observations have been made on normal C57-BL/6J and staggerer mutant mice. Morphological observations have given evidence it existed various neurones modifications which affected the olfactory bulb in the mutant mice. Olfactory bulb electrocorticograms (ECoG) of mutant have shown rare bursts of potentials of longer time duration than in normal mice.

The synthesis of vitellogenins by the digestive gland of Helix aspersa: evidence from cell-free translation of mRNA.

All the RNAs were extracted separately from the gonads and digestive glands of the garden snail Helix aspersa. After separation by oligo-dT-cellulose chromatography, the mRNA were evaluated using optical density measurements and agarose gel electrophoretic analysis and then used in a cell-free translation system: a rabbit reticulocyte lysate added with 35S methionine. The in vitro synthesized proteins were characterized by immunoprecipitation with polyclonal antibodies which were raised against mature oocyte proteins.

[Development of a bioassay from gonadal cellular cultures of the snail Helix aspersa: influence of nerve ganglion extracts on the synthetic activity of the target cells].

To realize bioassays a primary culture method was carried out with dissociated cells from the garden snail gonads. ADN and protein syntheses of gonadal cells were estimated using the liquid scintillation method. The gonadal cells were obtained from either adults of Petits Gris and Gros Gris, or young Gros Gris.

Localization of yolk proteins and their possible precursors using polyclonal and monoclonal antibodies, in Helix aspersa.

Three major yolk proteins of 140, 100, 80 KD and a faint band of 440 KD were determined by gradient gel electrophoresis in the mature eggs of Helix aspersa. Polyclonal and monoclonal antibodies were raised against mature oocyte extracts. The binding sites of these rabbit and hybridoma antibodies with the different yolk protein components were identified with a combination of WESTERN blotting, ELISA, immunofluorescence and immunogold staining.

[Electrophoresis of native cardiac myosin in Anura amphibians].

Electrophoresis in non dissociating conditions of native cardiac myosin was adapted to the study of Amphibian myosin. Utilization of potassium ion has allowed to obtain a good separation of myosin isoenzymes. An evolution of isoenzymic composition of cardiac myosin during metamorphosis and aging in Xenopus laevis (Daudin) was observed.

[Detection of collagen by immunofluorescence during development of Xenopus (Xenopus laevis Daud.)].

Appearance of the different collagen types was studied during the clawed toad development from hatching to metamorphosis, using indirect immunofluorescence techniques. Type-like IV collagen of basement membranes was first seen in the notochord streath. Type I, type II were detected at the same time, followed by type III.

[Differentiation of the prostate in the snail Helix aspersa Müll].

The differentiation of the snail Helix aspersa prostate gland is studied in animals from one to six months old. After the presentation of organogenesis the primary stage of secretion (3 months) and a stage where the prostate gland shows abundant secretions (6 months) were described; but the oviduct is still indifferentiated. The ultrastructural analysis shows the formation of secretory and ciliated cells in epithelioid prostatic tubes.

[Effects of alpha-amanitin on the development of Xenopus (Xenopus laevis Daud.) heart in vitro].

The differentiation of Xenopus heart is studied in vitro, in the presence of alpha-amanitin. The results obtained depend on the concentration of the inhibitor, the length of treatment and the stage of primordium. The mRNA pool assures the differentiation of explants, removed from young stages, for 12 hours.

[Ulstrastructure of Xenopus heart primordia, differentiated in culture in the presence of actinomycin D].

The cellular differentiation of Xenopus laevis cardiac primordia, developed in vitro with actinomycin D, was studied in fine structure. The ultrastructural changes appeared in mitochondria, and in nucleus, which showed a nucleolar characteristic segregation.

[Influence of actinomycin D on Xenopus heart differentiation in organ culture].

The cardiac differentiation of the African clawed Toad was studied in culture with Actinomycin D. In terms of drug amounts used and the duration of treatment, a limit coefficient was defined, beyond which the heart did not again differentiate. The results were interpreted by the existence of a threshold in the inhibition of ARNr synthesis, whose total turn-over seemed to be 24 hours.

Yes, you can teach clinical skills in the classroom!

This paper describes the use of videotaped interviews with volunteer patients as a method of teaching interviewing skills to first and second year occupational therapy students. It is hoped that this experience will enable students to conduct interviews in clinical or community settings with greater confidence and effectiveness.

[Development of heart innervation in Xenopux tadpole and relationship with organ function].

Cardial innervation is studied through specific techniques. It is set up in 9 to 18 mm tadpoles. The rhythm of the aneural heart progressively increases.