Comparative study on the in vitro and in vivo activities of heparinoids derivative investigated on the animal model.

In this study we compared the antithrombotic and anticoagulant properties of sodium and calcium derivatives of pentosan polysulfate (Na-PPS, Ca-PPS), unfractionated heparin (UFH), and low-molecular-weight heparin (Fraxiparin). The antithrombotic effects of these agents have been investigated in an experimental thrombosis model in which rat mesenteric venules with a diameter of 20-30 microm were injured by well-defined argon laser lesions. Furthermore, the in vivo and in vitro anticoagulant activities [activated partial thromboplastin time (aPTT), Heptest] of these agents have been studied.

Experimental studies on the anticoagulant and antithrombotic effects of sodium and calcium pentosan polysulphate.

In the present study we have compared the antithrombotic and anticoagulant properties of sodium and calcium derivatives of pentosan polysulphate (Na-PPS, Ca-PPS). The antithrombotic effect of these agents have been investigated in an experimental thrombosis model in which rat mesenteric venules diameter of 20-30 microm were injured by well defined Argon laser lesions. Furthermore, the in vivo and in vitro anticoagulant activities (aPTT, Heptest) of these agents have been studied.

Low molecular weight heparin versus acenocoumarol in the secondary prophylaxis of deep vein thrombosis.

The aim of this study was to determine the efficacy and safety of subcutaneous weight-adjusted dose low molecular weight heparin (LMWH) compared with oral anticoagulant (OA) in the prevention of recurrent venous thromboembolism. In a prospective multicenter trial, 202 patients with symptomatic proximal deep vein thrombosis (DVT) were included. As soon as the diagnosis of DVT was confirmed by phlebography, 101 were randomly assigned to receive LMWH (nadroparin) for secondary prophylaxis and 101 to receive OA (acenocoumarol).

The role of epidermal growth factor in platelet-endothelium interactions.

The objective of this study was to determine whether endogenous EFG released after submaximal physical exercise, affects platelet-endothelium interactions. Sixteen healthy male volunteers, aged 23-26 years, were submitted to a submaximal bicycle ergometry test. Blood for determination of EGF concentrations, platelet function studies (concentrations of beta-TG, PF4 and TXB2) and endothelium activity (LTC4 and endothelin-1,2 concentrations) was taken via an intravenous catheter before starting exercise and 15, 30 and 60 min after.

[Case report presentation for treatment of pure red cell aplasia].

We presented a case of a 34 year old male patient with pure red cell aplasia. He was treated with antithymocyte globulin (Pasteur Merieux, France) at a dose of 175 mg in 350 ml 0.9% NaCl in intravenous drip infusion--10 drip/min for 5 consecutive days.

Release of human monocytes protein C and the cofactor protein S in vitro.

Monocytes may contribute to a coagulation process by expression of the tissue factor and by synthesizing factors V, VII, X and XIII. Cultured blood monocytes also express both the tissue plasminogen activator and the plasminogen activator inhibitor I. The present study assesses the ability of human monocytes to secrete protein C and its cofactor protein S, which are potent inhibitors of the clotting cascade.

[Examination of interleukin levels and growth factor from blood platelets in leukemia].

Plasma concentrations of interleukines 1, 3, 6, 8 and platelet derived growth factor (PDGF) were estimated in 45 patients with leukaemias. Among patients were 12 with acute myeloblastic leukaemia-AML (type M1 according to FAB classification), 9 with chronic granulocytic leukaemia-CGL, 10 with blastic crisis of CGL (CGL-BC) and 14 with chronic lymphocytic leukaemia-CLL (in 1 and 2 stage according to Rai classification). Additionally the concentration of IL-3 was measured in 10 patients with exacerbation of CLL.

Antithrombotic and thrombolytic activity of heparins in a rat model of laser-induced thrombosis.

The mode of action by which heparins exert their antithrombotic effect is not fully understood. Antithrombotic action persists much longer than the inhibition of even sensitive coagulation parameters. We therefore have investigated antithrombotic and thrombolytic activity of two low molecular weight heparins Fraxiparin (Sanofi) and Clexane (Rhone-Poulenc) in a rat model of laser-induced thrombosis.

Effect of troxerutin on laser-induced thrombus formation in rat mesenteric vessels, coagulation parameters and platelet function.

The antithrombotic effect of Troxerutin have been studied in an experimental model of thrombosis in which rat mesenteric vessels (arterioles and venules) 25-30 microns in diameter were injured by well defined argon laser lesions. Furthermore in vitro effect of this agent on coagulation parameters (IIa, Xa inhibition, TT, heptest), and platelet function (platelet adhesion to the siliconised glass and extracellular matrix, platelet spreading) has been investigated 2 h after oral drug administration. Troxerutin at a dose of 10 mg/kg markedly inhibited thrombus formation in venules.

Effect of nonionic and ionic contrast media on fibrinolysis in patients undergoing angiography.

Radiologic contrast media may influence processes of hemostasis resulting in increased thrombotic or bleeding tendency. A number of clinical case reports suggest that the use of nonionic contrast media is associated with thrombotic complications. In vitro studies have indicated that nonionic contrast media may induce generation of thrombin in blood whereas ionic contrast agents do not show such an effect.

Interaction between the sulfated lactobionic acid (LW 10082) and other antithrombotic agents in animal thrombosis model.

We investigated the comparative antithrombotic properties of LW 10082, acetylasalicylic acid (Aspisol), low molecular weight heparin (LMWH, CY 216) and sodium heparin using laser-induced thrombosis model. The animal model which has been used is well suited to observe platelet reactions to damaged endothelial cells and also to evaluate the effect of drugs on the formation of platelet thrombi. The rat mesenteric venules with a diameter of 20-30 microns were injured by well defined argon laser lesion.

Prothrombin activation fragment 1 + 2 and thrombin-antithrombin-III complexes in plasma of patients with essential arterial hypertension.

Prothrombin activation fragment 1 + 2 (F1 + 2) and thrombin-antithrombin-III complexes (TAT) were measured in plasma of 34 patients with newly diagnosed essential hypertension. The patients with hypertension showed an increase in both F1 + 2 and TAT concentrations. The obtained results point to the intravascular thrombin generation in very early stages of essential hypertension.

Immunohistochemical demonstration of fibrin II in gastric cancer tissue.

Immunohistochemistry was applied to AMeX-fixed sections of twelve cases of gastric carcinoma obtained at surgical resection to explore the occurrence and distribution of fibrin deposits in situ. Fibrinogen was distributed in abundance throughout perivascular zones and in the connective tissue of the tumor stroma. Fibrin II (des-fibrinopeptide B-type fibrin) was easily identified in a direct apposition to the surface membranes of viable carcinoma cells, predominantly at the host-tumor interface and in the regions immediately adjacent to the zones of angiogenesis.

[Tissue plasminogen activator and plasminogen activator inhibitor in aqueous humor].

Background: In the anterior segment of the eye, fibrin clots must be rapidly resorbed to prevent fibrosis. Tissue-type plasminogen activator (t-PA), a serine protease that catalyzes the conversion from plasminogen to plasmin, plays an important role in the fibrinolytic system and has therefore in recent years attracted attention in the field of ophthalmology.

Material And Methods: The aqueous humor of patients undergoing cataract surgery was analyzed for the presence of components of the fibrinolytic cascade.

The role of monocytes in the platelet release of von Willebrand factor.

Circulating blood monocytes, which are a source of foam cells contribute to the vascular lesions. Von Willebrand factor (vWF) is an adhesive multimeric glycoprotein that is synthesized only by endothelial cells and megakaryocytes. In the present study we assessed the ability of human platelets to secrete vWF after incubation with blood monocytes in vitro.

Effects of 0-/-B-hydroxyethyl/rutoside on platelet function and thrombus formation in rat mesenteric vessels.

0-/-B-hydroxyethyl/rutoside has been investigated for its effect on laser-induced thrombus formation in rat mesenteric venules and arterioles. The in vitro effect of this agent on platelet adhesion to bovine subendothelial extracellular matrix (ECM) and glass, on spreading and on platelet aggregation induced by ADP, collagen and epinephrine have also been studied. The animal investigations of 0-/-B-hydroxyethyl/rutoside showed an antithrombotic effect in doses between 5 and 50 mg/kg after i.

Stimulated monocytes release factor XIII subunit A and fibronectin in vitro.

Factor XIII catalyses the formation of covalent bonds between several proteins involved in wound healing. Subunit A of factor XIII has been found inside and on the surface of monocytes and macrophages. In our study we investigated the ability of cultured monocytes to release factor XIII A subunit and its substrate-fibronectin.

Von Willebrand factor antigen in assessment of vasculitis in patients with connective tissue diseases.

Von Willebrand factor antigen (vWF:Ag) is synthesized and secreted by endothelial cells. In the present study we tried to assess the relationship between plasma level of vWF:Ag and vascular damage in patients with vasculitis. The study was carried out on 59 patients with connective tissue diseases.

[Antithrombotic properties of heparin independent of its anticoagulant activity].

Antithrombotic effects of two glycosaminoglycans: LMWH-Fraxiparin (Sanofi) and UFH Heparinum (Polfa) have been studied in the laser-induced rat thrombosis model. The investigations were carried out on male Wistar rats. Thrombus formation was induced in small mesenteric venules 25-30 microns in diameter using argon laser.

The antithrombotic effect of thromboxane receptor antagonist HN 11500 on thrombus formation in laser thrombosis model and platelet function tests.

The effect of thromboxane receptor antagonist HN 11500 (Chemie Linz AG, Austria) on thrombus formation in laser thrombosis model and platelet function test has been studied. In this study HN 11500 was a potent antithrombotic agent significantly inhibiting thrombus formation in venules in doses higher than 5 mg/kg after i.v.

Some haemorheological factors and transcutaneous PO2 in patients with a peripheral arterial occlusive disease after treatment with naftidrofuryl.

The aim of the present study was to investigate the influence of long term intravenous administration of naftidrofuryl (Dusodril-Lipha Arzn) twice daily in a dose of 200 mg in continuous, 4-hour infusion in 500 ml 0.9% NaCl to the patients suffering from a peripheral arterial occlusive disease (PAOD) in a clinical condition with special attention paid to transcutaneous partial oxygen pressure measurements (tcPO2) and rheographic parameters. Also the effect of treatment with naftidrofuryl on the platelet adhesion to the bovine extracellular matrix, the leukocyte adhesion to nylon fibres, and the number of platelet-leukocyte aggregates has been studied.

[Antithrombotic effects of molsidomine, isosorbide dinitrate and verapamil in a laser-induced thrombosis model].

Antithrombotic effects of molsidomine, isosorbide dinitrate and verapamil obtained from the Casela Riedel Pharma GmbH, Frankfurt/Main, Germany have been studied in the laser-induced rat thrombosis model. The investigations were carried out on male Wistar rats weighing 200-300 g. Thrombus formation was induced in small mesenteric arteries--25-35 microns diameter using argon laser.