Unlabelled: is to study the possibility of applying commercial wound coatings for treating infected wounds as a carrier matrix for bacteriophages.
Materials And Methods: Twelve varieties of commercial wound coverings based on biopolymers of natural and synthetic origin, a biological preparation Staphylophag produced by scientific-industrial association Microgen (Russia), registration certificate P N001973/01, and the 3196 test strain (GenBank JARQZO000000000) isolated from a patient with a burn wound have been used in our work. The ability of commercial biological wound coatings to absorb solutions was examined by immersing them in a physiological solution (pH 7.
Unlabelled: was to analyze the genome features of the probiotic strains 379, 1, and 791 and study their antiviral activity.
Materials And Methods: Whole genome sequencing of three strains of bifidobacteria was performed on the MiSeq platform (Illumina Inc., USA).
Sovrem Tekhnologii Med
March 2022
Unlabelled: was to study the species composition of colon microbiocenosis in patients with chronic kidney disease receiving programmed hemodialysis treatment and to evaluate the efficacy of its correction using a new immobilized synbiotic.
Materials And Methods: Samples of colon microbiota from 62 patients undergoing programmed hemodialysis were studied before and after a course of diet therapy that included probiotic components, in particular, the immobilized synbiotic LB-complex L. Isolation of microorganisms was carried out according to our original method; for bacteria identification, a MALDI-TOF Autoflex speed mass spectrometer (Bruker Daltonik, Germany) was used in the Biotyper program mode.
Purpose: The aim of this work was to analyze the complete genome of probiotic bacteria 8 RA 3, 90 TC-4, 39, 791, 1, and 379 and to test their activity against influenza A and SARS-CoV-2 viruses.
Methods: To confirm the taxonomic affiliation of the bacterial strains, MALDI TOF mass spectrometry and biochemical test systems were used. Whole genome sequencing was performed on the Illumina Inc.
When studying the effect of the metabolic products of clinical isolates of enterococci on the viability of Candida albicans, it was found that metabolites of all tested strains of Enterococcus faecium, E. faecalis had a fungistatic effect. At the same time a reliable fungicidal effect is a strain-specific feature.
View Article and Find Full Text PDFIn this study, activated carbon (AC) particles were combined with AZ91 alloy to manufacture a magnesium syntactic foam. This novel lightweight foam has a very low density, in the range of 1.12⁻1.
View Article and Find Full Text PDFWith the use of whole genome sequencing, the taxonomic status of Lactobacillus fermentum 90 TC-4 strain from Russian collections were studied. Complex analysis of phenotypical and genetic properties was conducted using phenotypic and molecular genetic methods. The main characteristics of the genome and biochemical activity profile of the strain were determined.
View Article and Find Full Text PDFFor the plant species, which is considered a short-lived perennial, we have composed a scale of ontogenetic stages and the life cycle graph (LCG) according to annual observations on permanent sample plots in an Alpine lichen heath during the 2009-2014 period. The LCG that reflects seed reproduction has been reduced to the one that avoids the stage of soil seed bank, yet preserves the arcs of annual recruitment. The corresponding matrix model of stage-structured population dynamics has four stages: juvenile plants (including seedlings), virginal, generative, and 'terminally generative' (the plants die after seed production).
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
June 2016
Aim: Confirmation of taxonomic position of Lactobacillus fermentum 90 TC-4 strain using phenotypic (classic microbiological, MALDI TOF mass-spectrometry) and genetic (16S rRNA gene segment sequencing and full genome sequencing) methods.
Materials And Methods: Object of the study--Lactobacillus fermentum 90 TC-4 strains from various collections. Mass-spectrometric analysis was carried out using Autoflex MALDI TOF mass-spectrometer (Bruker Daltonics, Germany), study of biochemical properties of the strain was carried out using API 50 CHL strips (Biomerueux, France), "DNA-sorb B" kitwas used for isolation ofgenome DNA (CRIE, Moscow).
The task to estimate the fitness of a clonal plant with polyvariant ontogeny reduces to compiling a life cycle graph, constructing and calibrating the corresponding matrix model of the discrete-structured population, and calculating the dominant eigenvalue (λ1) of the model matrix. We demonstrate a solution to this task with a sample of Calamagrostis epigeios , a perennial long-rhizome grass propagating vegetatively, and data on the age-stage structure of its local population. A traditional technique of successive censuses fixing the age-stage status of all individuals on a permanent sample plot (SP) provides for calculating frequencies of ontogenetic transitions directly from the data, but leaves uncertain the status-specific reproduction rates as the recruit parents are unknown (“reproductive uncertainty”).
View Article and Find Full Text PDFPolyvariant ontogeny (PVO) is expressed visually in the life cycle graphs (LCGs) for Calamagrostis woodreeds as a variety of pathways for individual plants to develop through many of their states distinguishable by the ontogenetic stage and chronological age (in years). PVO is recognized as the basic mechanism of adaptation in local plant populations to their environments, while they find a quantitative measure of the adaptation via developing a matrix model of double-structured population, calibrating the matrix of vital rates from empirical data, and calculating its dominant eigenvalue λ1. This approach encounters an obstacle typical for rhizome grasses: while the rates of aging and ontogenetic transitions can be deterinined from field data mainly by the morphology of aboveground parts of the plant, the rates of vegetative propagation can be reliably determined only from digging up the belowground rhizome system, i.
View Article and Find Full Text PDFThis paper addresses the computation of the effective diffusivity in new bioactive glass (BG) based tissue engineering scaffolds. High diffusivities facilitate the supply of oxygen and nutrients to grown tissue as well as the rapid disposal of toxic waste products. The present study addresses required novel types of bone tissue engineering BG scaffolds that are derived from natural marine sponges.
View Article and Find Full Text PDFJ Mater Sci Mater Med
November 2014
Tissue engineering scaffolds are designed to support tissue self-healing within physiological environments by promoting the attachment, growth and differentiation of relevant cells. Newly formed tissue must be supplied with sufficient levels of oxygen to prevent necrosis. Oxygen diffusion is the major transport mechanism before vascularization is completed and oxygen is predominantly supplied via blood vessels.
View Article and Find Full Text PDFZh Vopr Neirokhir Im N N Burdenko
May 2015
Unlabelled: Intramedullary spinal cord tumors (IMSCTs) are a rare neurosurgical disease. There are a lot of controversies in their epidemiology. Most references were published in the 80-90ss.
View Article and Find Full Text PDFJ Nanosci Nanotechnol
October 2012
Recently, using molecular dynamics simulation in conjunction with an embedded-atom method potential, we have predicted Pd2Ni surface-sandwich ordering at the nanoscale. These findings open up a range of opportunities for the synthesis of new kinds of Pd-Ni nanostructures such as a five-layer Pd2Ni nanofilm from which a Pd2Ni nanotube might be fabricated. In this paper, we report on an ab initio spatial optimization and structural energy calculation of a five-layer Pd2Ni nanofilm, which are performed using plane-wave pseudopotential total energy calculations in the generalized gradient approximation of density functional theory.
View Article and Find Full Text PDFZh Nevrol Psikhiatr Im S S Korsakova
April 2013
We studied 479 epileptic patients. The study included the evaluation of anamnesis, clinical and neurological examination, routine EEG and/or video-EEG-monitoring, MRI of the brain. The period of observation was 1-8 years (on average 2.
View Article and Find Full Text PDFZh Obshch Biol
December 2011
Whatever popular the slogan of nonlinear ecological interactions has been in theory, practical ecology professes the projection matrix paradigm, which is essentially linear, i.e., the linear matrix model paradigm for discrete-structured population dynamics.
View Article and Find Full Text PDFZh Nevrol Psikhiatr Im S S Korsakova
June 2011
Causes of losing the pharmacological remission with duration of more than one year have been analyzed in 220 patients with epilepsy. The most often cause of losing control of seizures was switching from brand antiepileptic drugs to generic analogue (60,4% of patients) with 28,2% of them being switched to topiramate generics. The results of switching of 160 patients from the brand form of topiramate (topamax) to its generics were compared to those in the control group of 52 patients who continued to receive the original form.
View Article and Find Full Text PDFSurface segregation in (110) B2-NiAl film approximately 3 nm thick is investigated by using molecular dynamics simulation with a reliable embedded-atom potential. The simulation is performed for the stoichiometric composition at a temperature of 1500 K, just below the melting temperature of the film model. It is found that the (110) surface is structurally stable but develops adatoms, vacancies and antisites.
View Article and Find Full Text PDFThe results of long-term studies on the transmission of the mode of asexual reproduction through seeds to maize from gamagrass, a closely related wild plant, performed in the Laboratory of Plant Cytology and Apomixis are summarized. The first apomictic hybrids between Zea mays and Tripsacum dactyloides were obtained in this laboratory more than 40 years ago and have been maintained until the present time. Cytogenetic studies on the hybrids have shown that at least nine chromosomes of the wild parent are necessary for the expression of asexual reproduction through seeds.
View Article and Find Full Text PDFZh Nevrol Psikhiatr Im S S Korsakova
December 2010
One hundred and sixty-one patients with the diagnosis of "posttraumatic epilepsy" have been studied. A study of anamnesis, a clinical and neurological examination, routine EEG and/or video-EEG-monitoring, MRI of the brain have been performed. The period of observation was 1-5 years (on average 3 years).
View Article and Find Full Text PDFZh Nevrol Psikhiatr Im S S Korsakova
May 2010
One hundred and thirty-eight patients (76 women and 62 men, aged from 16 to 65 years) with symptomatic partial epilepsy (n=128) and idiopathic generalized epilepsy (n=10) have been studied. All patients received keppra on regiments of monotherapy (n=30) and polytherapy (n=108). The study included evaluation of anamnesis, clinical and neurophysiological examination, routine EEG and/or video-EEG-monitoring, MRI of the brain, assessment of effectiveness, tolerability and quality of life (QUALIE-31, version 1).
View Article and Find Full Text PDFFive hundreds and thirty-six patients, aged from 19 to 50 years, have been studied. Occipital lobe epilepsy (OLE) have been diagnosed in 22 (4,1%) of cases. The study included evaluation of anamnesis, clinical and neurological examination, routine EEG and/or video-EEG monitoring and MRI of the brain.
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