Defective protein synthesis of S-30 fraction of reticulocytes of anemic b/b rats.

The preincubated S-30 fraction from reticulocytes of anemic b/b rats is not able to promote the synthesis of proteins in the presence of hemoglobin RNA provided either from the reticulocytes of phenylhydrazine-treated rats or from the reticulocytes of anemic b/b rats. It was observed that poly(U)-directed polyphenylalanine synthesis is negligible in the same system, suggesting the existence of some alteration in regulatory machinery for protein synthesis in the reticulocytes of the anemic b/b rats.

Formation of initiation complex with lambdamRNA in vitro.

The product from the in vitro transcription of native lambdaDNA by purified E. coli RNA polymerase does not stimulate total amino acid incorporation although it can program ribosomes for the first peptide bond synthesis in the presence of the three initiation factor A, B and C.