Animal nutrition and breeding conditions modify the physiology of isolated primary cells.

Animal primary cell cultures are widely used in biomedical research to investigate cell metabolism, diseases and to devise novel treatments. Modern animal breeding techniques are developed to unify, control and reduce the amount of microorganisms that the animals are being exposed to. Furthermore, health monitoring and strict caging and handling protocols allow animals to be exposed only to a selected spectrum of microbes.

Thoracic radionecrosis following repeated cardiac catheterization.

Radiodermatitis is a known complication in patients having undergone radiotherapy. It usually appears 2 to 5 years after irradiation. We are reporting on a case of radiodermatitis that occurred within months after coronary dilatation and stenting.

DMSO modulates the pathway of apoptosis triggering.

We demonstrate here that distribution of caspase-9 influences the pathway of apoptosis triggering, since caspase-9 is activated efficiently only when it is distributed solely in the cytosol. Caspase-9 moves to the nuclei in a response to cell stress during isolation of primary hepatocytes; this is called preapoptotic cell stress response. The dimethyl sulfoxide (DMSO) treatment cannot prevent the migration of caspase-9 into the nuclei when it is added to primary hepatocytes immediately after isolation; however, it can trigger redistribution of caspase-9 from the nuclei into the cytosol when added 1 day post-isolation.

Preapoptotic cell stress response of primary hepatocytes.

Unlabelled: Primary hepatocytes are an important in vitro model for studying metabolism in man. Caspase-9 and Bcl-2-associated X protein (Bax) are regulators of the apoptotic pathway. Here we report on the translocation of procaspase-9 and Bax from cytoplasm to nuclei as well as on dispersion of mitochondria; these processes occur after isolation of primary hepatocytes.

Effect of aminophylline on aspirin penetration into the central nervous system in rats.

This study investigated with the effect of aminophylline on the penetration of aspirin through the blood-brain barrier (BBB) into the central nervous system (CNS) in rats. Acetylsalycylic was injected into the right axillary artery, to avoid the drug affecting the peripheral organs before it reached the CNS. The test animals received subcutaneously (s.

Effect of caffeine on quinidine transport to the central nervous system in rats.

We studied the effect of caffeine on the transport of quinidine through the blood-brain barrier (BBB) to the central nervous system (CNS) in rats. The anesthetized animals received quinidine in the form of a retrograde intra-arterial bolus injection (15 s) into the right axillary artery 30 min after receiving a subcutaneous injection of caffeine (test group) or physiological solution (control group). Rats were decapitated at 30, 60, 90, 120, and 240 s after quinidine administration.

Remote controlled equipment for multiple blood withdrawal in gravitational physiology experiments.

Electro-mechanical equipment for multiple blood withdrawal from small experimental animals applied to a centrifuge with maximal 6g gravitational overloading has been developed and tested. The equipment consists of a transmitter and receiver equipped by microcomputers. Active rotor stepping motors are driving four pairs of syringes.

Evidence for spinal cord hypersensitivity in chronic pain after whiplash injury and in fibromyalgia.

Patients with chronic pain after whiplash injury and fibromyalgia patients display exaggerated pain after sensory stimulation. Because evident tissue damage is usually lacking, this exaggerated pain perception could be explained by hyperexcitability of the central nervous system. The nociceptive withdrawal reflex (a spinal reflex) may be used to study the excitability state of spinal cord neurons.

[Professional and social responsibilities of clinical pharmacology].

According to the decision of the Scientific and educational board of the Medical Faculty in Novi Sad the Institute for pharmacology toxicology and clinical pharmacology introduced the clinical pharmacology in 1975. Postgraduate studies were organized for those wishing to specialize, i.e.

Effect of the aluminium ion on hepatic elimination of quinine and quinidine.

A study of the influence of the aluminum ion on the blood and hepatic kinetics of two alkaloid stereoisomers--quinine and quinidine--after their p.o. and s.

[Medicines and lactation].

The factors that determine the transfer of medicine from mothernal blood to breast milk are the relative molecular mass of the substance, liposolubility, plasma half-life, binding to plasma proteins, pKa, the rate of metabolism and the dose taken by the breast feeding mother. The transfer of large number of medicines is small, so that the amount reaching the infant is negligible. But, certain drugs pass the milk into quantities sufficient to cause adrverse effects and these drugs should be avoided during breast-feeding.

[The value of local administration of antibiotics in treatment of bone infections].

An open, controlled, randomized clinical investigation was carried out in 33 patients suffering from osteomyelitis. In the first group, 17 patients, a through drainage with sterile physiologic solution was applied, while in the second group, 16 patients, antibiotic was added to the sterile physiologic solution. In all patients values of C reactive protein (CRP) in the blood were examined, and later on every third day after the operation.

The effect of aluminium chloride upon the transition of drugs through the blood-brain barrier into the central nervous system.

In order to determine the effect of Al3+ upon the transition of drugs through the blood-brain barrier into the central nervous system we examined its effect upon a drug that dissociates as a cation (quinidine) and drugs that dissociate as anions (acetylsalicylic acid and pentobarbital). The entry and exit of quinidine into and out of the brain in mice pre-treated with AlCl3 was inhibited. Al3+ did not compete with acetylsalicylic acid for the penetration through the blood-brain barrier but did slow down its elimination from the brain.