Differences in serum levels of CB-153 and p,p'-DDE, and reproductive parameters between men living south and north in Norway.

Arctic is contaminated with persistent organochlorine pollutants (POPs), and exposure to these compounds may differ between south and north in Norway. POPs may have negative impact on male reproductive characteristics. We compared serum levels of the CB-153 and p,p'-DDE in men who were born and had lived most of their lifetime south and north (close to or above the Arctic Circle) in Norway.

Association between tobacco exposure and reproductive parameters in adolescent males.

Cigarette smoking is quite prevalent in the general population but our knowledge of its effect on male reproductive function is still very limited. Therefore, we investigated the impact of tobacco exposure on reproductive characteristics in young males. Military conscripts, 217 non-smokers and 85 smokers, with a median age of 18 years were enrolled.

Reproductive hormone levels in men exposed to persistent organohalogen pollutants: a study of inuit and three European cohorts.

Objective: Persistent organohalogen pollutant (POP) exposure may have a negative impact on reproductive function. The objective of this study was to assess the impact of POP exposure on the male hypothalamo-pituitary-gonadal axis.

Participants: Participants included 184 Swedish fishermen and spouses of pregnant women from Greenland (n = 258), Warsaw, Poland (n = 113) , and Kharkiv, Ukraine (n = 194).

Reproductive function during summer and winter in Norwegian men living north and south of the Arctic circle.

Seasonal, daylight-dependent variation in human spermatozoa counts, with lowest values during summer, has been suggested. To test this hypothesis, we performed a longitudinal study of semen quality and reproductive hormone levels in Norwegian men living north and south of the Arctic Circle. An ejaculate and a serum specimen were obtained both in summer and in winter from 92 volunteers in Tromsoe (69 degrees north latitude) and 112 in Oslo (60 degrees north latitude).

Serum levels of 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) in relation to markers of reproductive function in young males from the general Swedish population.

A time-related deterioration in male reproductive function caused by exposure to endocrine disrupters, including persistent organochlorines (POCs), has been hypothesized. In animal studies, POCs were found to have adverse effects on male reproductive function. However, little is known about the impact of POC exposure on reproductive parameters in men.

Susceptibility factors and DNA adducts in peripheral blood mononuclear cells of aluminium smelter workers exposed to polycyclic aromatic hydrocarbons.

Formation of DNA adducts as a result of exposure to polycyclic aromatic hydrocarbons (PAH) was studied in 98 potroom workers from an aluminium smelting plant and in 55 blue-collar workers without occupational PAH exposure. DNA from peripheral blood mononuclear cells (PBMC) was used for quantitation of individual PAH-DNA adducts by 32P-postlabelling/high performance liquid chromatography (HPLC) analysis. Four individual DNA adducts (denoted A, B, C and D) were quantified in 141 of a total of 153 subjects.

High consumption of fatty fish from the Baltic Sea is associated with changes in human lymphocyte subset levels.

Fatty fish from the Baltic Sea accumulate immunotoxic persistent organochlorine compounds. In a previous study we found inverse correlations between such fish consumption and natural killer (NK) cell levels in a Swedish population. The present study concerns 68 Latvian subjects with high, low or intermediate fish consumption.

Aromatic DNA adducts, micronuclei and genetic polymorphism for CYP1A1 and GST1 in chimney sweeps.

Aromatic DNA adducts in total white blood cells, cytochrome P450 (CYP) class 1A1 and glutathione transferase (GST1) class mu genotypes and micronuclei in T- and B-lymphocytes were studied in 69 full-time chimney sweeps and 35 controls, all male subjects. The sweeps had a higher (22%) but statistically non-significant increase in the level of DNA adducts as compared to the controls when all individuals independent of genotype were compared. The non-inducible CYP1A1 genotype, m1/m1, lacking a MspI restriction site at the 3' end of the gene, was associated with significantly higher adduct levels in the sweeps.

Diagnostic accuracy of fine needle aspiration cytology in pancreatic lesions. A review of 77 cases.

Fine needle aspiration cytologic specimens from pancreatic lesions in 77 patients were blindly reviewed. The patients were divided into two subgroups: group A included 19 patients without malignancy of the pancreas, and group B included 58 patients with confirmed malignancy (primary or secondary) of the pancreas. The original cytologic diagnoses in both groups were plotted against the respective reevaluated cytologic diagnoses.

B- and T-lymphocyte micronuclei in chimney sweeps with respect to genetic polymorphism for CYP1A1 and GST1 (class Mu).

Epidemiological studies have shown an increased incidence of lung cancer, bladder cancer, and esophageal cancer in chimney sweeps, probably due to their exposure to PAH in soot. The work environment for sweeps has, however, improved during the last decades. It was thus important to assess whether the present exposure still may cause genotoxic effects.

Biological effects in a chemical factory with mutagenic exposure. III. Urinary mutagenicity and thioether excretion.

Eighty-five workers in a chemical plant, in which there was an increased frequency of B-lymphocyte tumours (myelomas and lymphomas) and lung cancer, did not excrete higher amounts of thioethers in urine than 48 control subjects. The chemically exposed workers had, however, a higher excretion of thioethers in urine during shift compared to an exposure-free period. The bacterial fluctuation test showed a statistically significantly increased mutagenic activity for E.

Biological effects in a chemical factory with mutagenic exposure. I. Cytogenetic and haematological parameters.

Analysis in two independent laboratories demonstrated no significant differences in chromosome aberrations or micronuclei in lymphocytes from peripheral blood between workers in a chemical factory (exposed to a mixture of chemicals, such as piperazine, low levels of ethylene oxide and formaldehyde, aromatic nitrogen compounds, and other aromatic compounds) compared to unexposed control subjects. The chemical workers had significantly higher counts of lymphocytes (3.57 vs.

Dissemination pattern of two immunologically induced transplanted malignant lymphomas in C57 B1/6J mice.

The need for syngenic tumors to elucidate factors in lymphoma dissemination prompted us to induce new lymphomas in a series of inbred C57B1/6J mice. The method of induction, immunosuppression and simultaneous immunostimulation, had a high mortality rate, but gave us 2 transplantable lymphomas. These tumors are described and compared in the present paper, with emphasis on their dissemination patterns.

Benign solitary neurilemoma (Schwannoma). A correlative cytological and histological study of 28 cases.

A correlative histological and cytological study of 28 cases of solitary neurilemoma is presented. The typical histological mixture of Antoni type A and B tissue correlated well with the cytological findings. In the smears, the Antoni type A tissue was represented by tissue fragments with a fibrillar ground substance and slender, spindle-shaped cells forming obvious Verocay bodies in 24 cases, and the Antoni type B tissue by loose, microcystic fragments with single, elongated nuclei sometimes looking like fish-hooks and with indistinct, slender, cytoplasmic processes.

Alteration of a tumor cell distribution pattern by cytochalasin B.

Cytochalasin B pretreatment of tumor cells alters the distribution pattern of metastases after i.v. injection.

In vitro effects of Cytochalasin B on TA3 tumor cells.

We studied the effect of Cytochalasin B (CB) on two lines of the ascites tumor TA3 by microcinematography and scanning electron microscopy. CB induces a dose dependent and reversible cell paralysis. One microgram CB/ml causes a pronounced but incomplete paralysis.

Tumor cell locomotion--a factor in metastasis formation? Influence of cytochalasin B on a tumor dissemination pattern.

The influence of Cytochalasin B (CB) on TA3 ascites tumor cells was studied in vivo in order to assess whether CB-induced cell paralysis would affect the transplanation behavior of the cells and in particular tumor distribution after IV cell infusion ("experimental metastases"). Tumor cell pre-treatment with CB (1 mug/ml) did not alter the SC or IV transplantability of TA3 cells. Pre-treatment with 10 mug/ml CB, in contrast, consistently increased the incidence and number of extra-pulmonary tumor takes from IV transfused cells.