Publications by authors named "B Zumkehr"
Background: Despite being first identified in 1947, Zika virus-related outbreaks were first described starting from 2007 culminating with the 2015 Latin American outbreak. Hypotheses indicate that the virus has been circulating in Asia for decades, but reports are scarce.
Methods: We performed serological analysis and screened placental samples isolated in 2008 for the presence of Zika virus from pregnant women in Ho Chi Minh City (Vietnam).
Article Synopsis
- The Omicron-BA.1 variant of concern became the dominant strain globally in early 2022, prompting the need for extensive research using primary cell cultures and animal models to understand its characteristics compared to the Delta variant.* -
- In laboratory studies, Omicron-BA.1 showed increased early replication in human nasal cells but less replication in bronchial cells; however, in animal models like hamsters and ferrets, Delta variant remained more dominant.* -
- The research revealed that the spike gene from Omicron-BA.1 leads to lower replication and pathogenicity in certain mice, while also indicating that this variant may escape immune responses generated by mRNA vaccines, contributing to its dominance over other variants.*
Here, we present a protocol to analyze the T cell profiles of the neonatal ovine lung during respiratory syncytial virus (RSV) infection. The protocol delivers standardized multiparameter flow cytometry (FCM) analysis of CD4, CD8, regulatory, and γδ T cells isolated from lung, lymph nodes, and bronchoalveolar lavages (BALs). We detail the preparation of RSV and transtracheal inoculation of newborn lambs.
View Article and Find Full Text PDFWe present a protocol to generate an advanced model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue.
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