Synthesis of p-aminohippuric acid analog and its labeling by technetium-99m.

A procedure is described for the synthesis of p-[(bisacarboxymethyl)aminomethyl carbamino] hippuric acid (PAHIDA). The compound was identified by i.r.

Chemical and biological properties of 2,6-diisopropyl IDA labelled with 99mTc.

A procedure is described for the synthesis of 2,6-diisopropyl IDA (DISIDA) and for preparation of the radiopharmaceutical by "instant technique". The chemical parameters affecting the formation of the Sn(II)-DISIDA complex were also determined. Biological studies involved lyophylic measurements, determination of binding constants as well as rate of binding to proteins and biodistribution.

Synthesis and quality control of 99mTc-p-butyl IDA.

A procedure is described for synthesis of p-butyl IDA and for identification of the product by i.r. and NMR spectroscopy and by chemical analysis.

Preparation and quality control of 99mTc-calciumgluconate.

A procedure for the labelling of lyophilised Sn-Ca-Gluconate by a 99mTc instant technique was developed. Paper and TLC chromatography and low-voltage electrophoresis were used for the determination of the labelling yield which reached between 95 to 98%. The biodistribution was followed in white (albino) rats.

Detection of acute myocardial infarction in spontaneously hypertensive rats by 99mTc-pyrrolidino methyl tetracycline.

The myocardial infarct induced by isoproterenol in spontaneously hypertensive rats accumulates higher activities of 99mTc-PM tetracycline compared with the cardiac infarct in normotensive rats caused by the same method. The isoproterenol model of the myocardial necrosis was induced in intact rats without opening the thorax and is a convenient method for experimental radioisotope studies.