A Modular Cloning Toolkit for the production of recombinant proteins in .

Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs. Here, a team of bachelor students established a MoClo toolkit for the protist in the frame of the international Genetically Engineered Machine (iGEM) competition. Our modular toolkit is based on a domesticated version of a commercial LEXSY expression vector and comprises 34 genetic parts encoding various affinity tags, targeting signals as well as fluorescent and luminescent proteins.

A Computed Tomographic Study of the Molar Teeth of spp.

A photographic and computed tomography (CT) scanning study was carried out on 295 molar teeth of 18 adult male skulls and 8 skulls of including seven adult males and one adult female. The occlusal morphology of the permanent maxillary and mandibular molar teeth of was very similar to that of . Most maxillary molar teeth had six roots, with small numbers of teeth having four, five or seven roots.

A Computed Tomographic Study of the Premolar Teeth of spp.

A photographic and computed tomography (CT) scanning study was carried out on the premolar teeth of 18 adult male skulls, 10 skulls of , including 6 adult males, 1 adult female, 1 subadult male, 1 subadult female, and 1 juvenile male. The occlusal morphology of the permanent maxillary premolar teeth of was very similar to that of . Almost all the maxillary third premolar teeth (107/207) had 2 roots, whereas maxillary fourth premolar teeth (108/208) had 3 or 4 roots.

The anatomy and histology of the reproductive tract of the male Babirusa (Babyrousa celebensis).

The anatomy and histology of the reproduction organs of the male Babirusa (Babyrousa celebensis) were studied in 21 specimens collected between birth and approximately 17 years of age. In adult animals the testes were located in a subanal position against the caudal surface of the thigh musculature. Average adult testis length was 68.

Anatomy and histology of the reproductive tract of the female Babirusa (Babyrousa celebensis).

The anatomy and histology of the female reproductive tract of the Indonesian wild pig Babyrousa celebensis was studied by means of reproductive tracts obtained from seven animals aged between two and 22 years of age. The ovary appeared to have the ability to ovulate up to four ova at one time. However, the combined ovarian output seemed to average 1.