Publications by authors named "B Zelezna"
Liver-enriched antimicrobial peptide 2 (LEAP2) is a natural antagonist/inverse agonist of ghrelin receptor GHSR. Its truncated palmitoylated analog palm-LEAP2(1-14) promised anti-obesity properties because it exhibited favourable stability and an acute anorexigenic effect in our previous studies. Here we demonstrate desirable palm-LEAP2(1-14) pharmacokinetics, with significant levels of the peptide persisting in mouse blood 3 h after its subcutaneous administration.
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- GPR10 and NPFFR2 receptors are crucial for controlling food intake and energy balance, and studying their ligands can help create treatments for obesity.
- Double knockout (dKO) mice, which lack both receptors, were tested on standard and high-fat diets to understand the metabolic effects of their deficiency, showing issues like glucose intolerance and insulin resistance.
- The dKO males gained more weight due to higher caloric intake, while dKO females showed obesity and anxiety-like behavior, highlighting the need to investigate these receptor pathways for anti-obesity therapies.
Aims: This study investigates the neuroprotective effects of lipidized analogues of 2-SS-CART(61-102) derived from anorexigenic neuropeptide cocaine- and amphetamine-regulated transcript peptide (CARTp) in light of the link between obesity, its comorbidities, and the development of Alzheimer's disease.
Methods: We introduce novel lipidized analogues derived from 2-SS-CART(61-102), a specific analogue of natural CART(61-102), with two disulfide bridges. Using hypothermic PC12 cells, we tested the effect of the most potent analogues on Tau phosphorylation.
Since 1975, the incidence of obesity has increased to epidemic proportions, and the number of patients with obesity has quadrupled. Obesity is a major risk factor for developing other serious diseases, such as type 2 diabetes mellitus, hypertension, and cardiovascular diseases. Recent epidemiologic studies have defined obesity as a risk factor for the development of neurodegenerative diseases, such as Alzheimer's disease (AD) and other types of dementia.
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- RF-amide peptides play a key role in various physiological processes including appetite regulation, stress response, behavior, and reproductive functions.
- The study focused on neuropeptide FF receptors (NPFFR1 and NPFFR2) by creating lipidized analogs of neuropeptide AF (NPAF) and a stable analog of neuropeptide FF, 1DMe, and testing their effects on food intake in mice.
- While acute administration of oct-NPAF and oct-1DMe reduced food intake in fasted mice, neither had long-term effects on food consumption or body weight in diet-induced obesity. However, oct-1DMe was linked to anxiety-like behavior and changes in hormone levels.