Publications by authors named "B Zegura"
In vitro hepatic 3D cell models and their application in genetic toxicology: A systematic review.
Mutat Res Genet Toxicol Environ Mutagen
December 2024
Article Synopsis
- The rising use of new chemicals and consumer products has spurred concerns about their potential to cause DNA damage and significant health issues, leading to a demand for genotoxicity testing data.
- Traditional testing methods involve using laboratory animals, which are expensive and raise ethical issues, prompting the development of more accurate in vitro approaches that minimize animal use.
- In particular, 3D cell models have emerged as promising alternatives to 2D cultures, providing better physiological relevance and predictive data for in vivo conditions, and their use in genotoxicity testing has been increasing, as highlighted in a review of 31 recent studies.
The feasibility and technical capacity for producing crosslinked sub-micron gels with a nano spray-dryer were studied with variable pH systems incorporating alginate, pectin, and pullulan. The obtained powders were characterized for their morphology, particle size distribution, and their toxicological safety profile using genotoxicity and cytotoxicity assays. Additionally, quercetin was added to the encapsulation system to study the potential of the system to encapsulate this material.
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- Cylindrospermopsin (CYN) is a toxic cyanotoxin that may enhance the toxicity of other contaminants like arsenic (As) and cadmium (Cd), prompting research into their combined effects on human immune cells.
- Tests showed that both As and Cd reduced the viability of immune cell lines Jurkat and THP-1, with specific exposure concentrations noted for each contaminant.
- The study also found that CYN, when combined with As or Cd, affected the expression of cytokines, indicating complex interactions that suggest a necessary reevaluation of risk assessments for environmental contaminants.
Background: The aim of this double-blind, placebo-controlled study was to investigate the effect of vitamin E supplementation as an addition to a commercial renal diet on survival time of cats with different stages of chronic kidney disease (CKD). In addition, we were interested whether vitamin E supplementation affects selected oxidative stress and clinical parameters. Thirty-four cats with CKD and 38 healthy cats were included in the study.
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