The Basic Psychological Need Satisfaction and Frustration Scale: Some Satisfying and Frustrating Findings.

As a tool for operationalizing basic psychological needs theory (an aspect of self-determination theory), the Basic Psychological Needs Satisfaction and Frustration Scale (BPNSFS) has become the primary self-report measure. Here, with a sample of 807 Canadian military members, we confirm the BPNSFS six content factor structure and, in supporting that structure, demonstrate the differential association of satisfaction and frustration scales with psychological functioning variables that are external to the factor analysis. Although the specific content interpretation of the BPNSFS can be debated, we support the continued use of the BPNSFS as a psychometrically sound instrument.

Perspective on the Potential Vertical Transmission of SARS-CoV-2 Through Breast Milk.

Aim: SARS-CoV-2 is highly transmissible, having infected ~16 million children in the United States. Symptom severity is higher in infants compared to older children, possibly due to their ineligibility for vaccination. Concerns persist that mothers transmit infectious viral loads of SARS-CoV-2 through breast milk.

Nothnagel Syndrome Without Extremity Symptoms: A Case Report.

The finding of pupil-sparing third nerve palsy is synonymous with diabetic third nerve palsy in the minds of many clinicians. While this is the most common cause of a third nerve palsy with normal pupillary response, it is not the only cause. We present the case of an elderly diabetic gentleman who presented with pupil-sparing third nerve palsy and gait abnormalities without any weakness or incoordination in the extremities.

An analysis of the structural changes of the oxygen evolving complex of Photosystem II in the S and S states revealed by serial femtosecond crystallography.

Photosystem II (PSII) is a unique natural catalyst that converts solar energy into chemical energy using earth abundant elements in water at physiological pH. Understanding the reaction mechanism will aid the design of biomimetic artificial catalysts for efficient solar energy conversion. The MnOCa cluster cycles through five increasingly oxidized intermediates before oxidizing two water molecules into O and releasing protons to the lumen and electrons to drive PSII reactions.

Exploring CRISPR-Cas9 HNH-Domain-Catalyzed DNA Cleavage Using Accelerated Quantum Mechanical Molecular Mechanical Free Energy Simulation.

The target DNA (tDNA) cleavage catalyzed by the CRISPR Cas9 enzyme is a critical step in the Cas9-based genome editing technologies. Previously, the tDNA cleavage from an active SpyCas9 enzyme conformation was modeled by Palermo and co-workers (Nierzwicki et al., , 912) using ab initio quantum mechanical molecular mechanical (ai-QM/MM) free energy simulations, where the free energy barrier was found to be more favorable than that from a pseudoactive enzyme conformation.