Publications by authors named "B W Bardoel"
Article Synopsis
- Staphylococcus aureus is a leading cause of severe healthcare-related infections, and existing antibiotic treatments often have high mortality rates, necessitating new treatment approaches.
- Researchers studied blood samples from 17 S. aureus bacteremia patients to analyze immune responses by isolating plasmablasts and sequencing their antibody genes, resulting in the identification of over 300 unique antibody sequences.
- Four novel monoclonal antibodies (mAbs) were developed, with one specifically targeting wall teichoic acid in S. aureus, while three showed cross-reactivity with Staphylococcus epidermidis and were able to trigger immune cell phagocytosis of staphylococci.
Antibody-dependent complement activation plays a key role in the natural human immune response to infections. Currently, the understanding of which antibody-antigen combinations drive a potent complement response on bacteria is limited. Here, we develop an antigen-agnostic approach to stain and single-cell sort human IgG memory B cells recognizing intact bacterial cells, keeping surface antigens in their natural context.
View Article and Find Full Text PDFThe Gram-negative bacterium Klebsiella pneumoniae is an important human pathogen. Its treatment has been complicated by the emergence of multi-drug resistant strains. The human complement system is an important part of our innate immune response that can directly kill Gram-negative bacteria by assembling membrane attack complex (MAC) pores into the bacterial outer membrane.
View Article and Find Full Text PDFTherapeutic bacteriophages (phages) are primarily chosen based on their in vitro bacteriolytic activity. Although anti-phage antibodies are known to inhibit phage infection, the influence of other immune system components is less well known. An important anti-bacterial and anti-viral innate immune system that may interact with phages is the complement system, a cascade of proteases that recognizes and targets invading microorganisms.
View Article and Find Full Text PDFAntibodies play a key role in the immune defence against Gram-negative bacteria. After binding to bacterial surface antigens, IgG and IgM can activate the complement system and trigger formation of lytic membrane attack complex (MAC) pores. Molecular studies to compare functional activity of antibodies on bacteria are hampered by the limited availability of well-defined antibodies against bacterial surface antigens.
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