A genetically encoded sensor for visualizing leukotriene B4 gradients in vivo.

Leukotriene B (LTB) is a potent lipid chemoattractant driving inflammatory responses during host defense, allergy, autoimmune and metabolic diseases. Gradients of LTB orchestrate leukocyte recruitment and swarming to sites of tissue damage and infection. How LTB gradients form and spread in live tissues to regulate these processes remains largely elusive due to the lack of suitable tools for monitoring LTB levels in vivo.

Predicting No Show in Voice therapy: Avoiding the Missed Appointment Cycle.

Introduction: Voice therapy plays a critical role in the treatment of voice disorders. Despite positive outcomes in patients who attend voice therapy, otolaryngologists, and speech-language pathologists continue to struggle with patient compliance. Previous studies evaluating the multidisciplinary clinic model have shown better completion, VHI-10 scores, and fewer cancelation and no-shows (NS).

[Diagnosis of cerebral hemorrhage before the era of computed tomography].

Introduction: During the past decades there has been a great progress in neuroimaging methods. Cranial computed tomography is part of the daily routine now and its use allows a fast diagnosis of parenchymal hemorrhage. However, before the availability of computed tomography the differentiation between ischemic and hemorrhagic stroke was based on patient history, physical examination, percutan angiography and cerebrospinal fluid sampling, and the clinical utility could be evaluated by autopsy of deceased patients.

Glucose concentration gradient through the cerebral cortex in normoglycaemic anaesthetized rabbits.

In this pilot study, the relationships between glucose and lactate concentrations of plasma, cerebrospinal fluid (CSF), cerebral cortex and subjacent white matter were investigated in one hyperglycaemic and three normoglycaemic anaesthetized rabbits. After a 90 min stabilization period, CSF was sampled and the brain frozen in situ. Triplet samples (n = 3 X 21) were obtained from the outer and inner halves of cortex and from the white substance and analysed for their water content as well as for glucose and lactate by enzymatic fluorescence methods.