[The purification and concentration of the rabies virus by a diafiltration concentration method].

Purified concentrates of rabies virus were prepared by both microfiltration chromatography and diafiltration methods. The diafiltration concentrate had a high level of protective activity and was not inferior to the microfiltration chromatographic one. The infectious activity was better retained after diafiltration, the chromatographic purity of both preparations was similar.

[Chemotactic function of skin fibroblasts in patients with amyloidosis].

Chemotaxis of cultivated fibroblasts, obtained from patients with amyloidosis, chronic glomerulonephritis and healthy volunteers, was investigated. Fibroblast migration toward donor serum and serum from patients with amyloidosis was measured using Boyden chamber's technique. As "zero" chemoattractant Hank's solution was used.

[The submolecular structure of the Carnation Mottle virus].

The model of the tertiary structure of the CMtV subunit was suggested and the mode of its packaging into the capsid was determined on the basis of electron microscopy small-angle X-ray scattering and X-ray diffraction data. The i-ray diffraction data on the crystal of the native virion of Carnation Mottle Virus were obtained on the automatic diffractometer supplied with two-dimensional detector.

[Use of microfiltration and gel filtration technics for purifying and concentrating the Venezuelan equine encephalomyelitis virus].

A system for purification and concentration of Venezuelan equine encephalomyelitis (VEE) virus omitting large-scale ultracentrifugation was developed. The first step consists in prefiltration through large pore nuclear filters (NF) to remove large particle admixtures from the virus-containing fluid. In the second step, the resulting filtrage undergoes concentrating microfiltration through small pore NF.

[Preparative isolation of myxovirus glycoproteins and the study of their immunogenic properties].

Preparative isolation of glycoproteins from ortho- and paramyxoviruses is described. The purified concentrated virus has been treated with nonionic detergent MESK with subsequent removal of viral cores by centrifugation. Supernatant was sterilized by filtration through the nuclear filters and cleared from detergent by dialysis.