Enhanced Tunability of Photo-Cross-Linkable Silk Sericins from .

Over the past decade, silk sericin has emerged as a promising material for biomedical applications, especially in tissue engineering, where fine-tuning the physicochemical properties is crucial. However, previous studies, including those on the methacrylation of sericin (yielding SS-MA), showed limited tunability. Here, we developed a photo-cross-linkable sericin-based material modified with 2-aminoethyl methacrylate (AEMA) using two synthesis routes: sequential modification of SS-MA with AEMA (SS-MA-AEMA) and an efficient one-pot synthesis (SS-AEMA).

Optical Nanomotion Detection to Rapidly Discriminate between Fungicidal and Fungistatic Effects of Antifungals on Single-Cell .

is an emerging pathogen that poses a significant challenge due to its multidrug-resistant nature. There are two types of antifungal agents, fungicidal and fungistatic, with distinct mechanisms of action against fungal pathogens. Fungicidal agents kill fungal pathogens, whereas fungistatic agents inhibit their growth.

Bubbling insights: unveiling the true sophorolipid biosynthetic pathway by Starmerella bombicola.

Background: The yeast Starmerella bombicola is renowned for its highly efficient sophorolipid production, reaching titers and productivities of (over) 200 g/L and 2 g/(L h), respectively. This inherent efficiency has led to the commercialization of sophorolipids. While the sophorolipid biosynthetic pathway has been elucidated a few years ago, in this study, it is revisited and true key intermediates are revealed.

Revision of the sophorolipid biosynthetic pathway in Starmerella bombicola based on new insights in the substrate profile of its lactone esterase.

Background: Sophorolipids (SLs) are a class of natural, biodegradable surfactants that found their way as ingredients for environment friendly cleaning products, cosmetics and nanotechnological applications. Large-scale production relies on fermentations using the yeast Starmerella bombicola that naturally produces high titers of SLs from renewable resources. The resulting product is typically an extracellular mixture of acidic and lactonic congeners.

Immobilization and docking studies of Carlsberg subtilisin for application in poultry industry.

Carlsberg subtilisin from Bacillus licheniformis PB1 was investigated as a potential feed supplement, through immobilizing on bentonite for improving the growth rate of broilers. Initially, the pre-optimized and partially-purified protease was extracted and characterized using SDS-PAGE with MW 27.0 KDa.