Correlation of nucleotides and carbohydrates metabolism with pro-oxidant and antioxidant systems of erythrocytes depending on age in patients with colorectal cancer.

Aim: To examine the relationship between metabolic features of purine nucleotides and antioxidant system depending on the age of patients with colorectal cancer.

Materials And Methods: The activity of adenosine deaminase, xanthine oxidase, glutathione peroxidase, superoxide dismutase and glucose-6-phosphate dehydrogenase, the NOx concentration and the oxidative modification of proteins were determined spectrophotometricaly in 50 apparently healthy people and 26 patients with colorectal cancer stage -III---IV, aged 40 to 79 years.

Results: Increase of pro-oxidant system of erythrocytes with the age against decrease in level of antioxidant protection in both healthy individuals and colorectal cancer patients was determined.

Activity of thymidilate "salvage pathway" enzymes in human gastric cancer and blood serum: correlation with treatment modalities.

Unlabelled: A comparative study of enzyme activity features of thymidilate "salvage pathway" synthesis in blood serum and tissues of different age patients with gastric cancer (T(3-4)N(0-x)M(0)) was carried out.

Aim: To evaluate the diagnostic relevance of thymidilate metabolism enzymes activities and their association with tumor growth.

Methods: Enzymes activities were determined by the radioisotope method and spectrophotometrically in tumor tissues and blood serum of 74 patients.

[Lactate dehydrogenase, adenosine deaminase and thymidine phosphorylase activity of blood and tissues in breast cancer].

Lactate dehydrogenase (LDH), adenosine deaminase and thymidine phosphorylase activity was analyzed in the blood serum, primary tumor and adjacent uninvolved breast tissues from 49 women with adenocarcinoma and from 10 ones with benign adenofibroma. The LDH activity was increased in both cancerous and adjacent tissues. Serum LDH level reflects cell membrane alterations not only in the tumor node cells but also to a greater extent--in the surrounding unmalignant tissues.

[Disturbed metabolism of gastric mucosa DNA precursors as prognosticator of neoplastic transformation of gastric ulcer].

Levels were assayed and compared of metabolic enzymes--thymidine and adenosine--in gastric mucosa tissues and blood serum from patients with gastric ulcer disease and gastric cancer (age--30-60 yrs). Enzymatic levels matched to the highest degree in cases of ulcer disease aggravated by chronic atrophic gastritis and precancerous alterations (dysplasia, metaplasia). Elevated concentrations of thymidine kinase and phosphorylase and adenosine desaminase were identified both at foci of ulceration (p > 0.

[The role of enzymatic features of hypoxia in ulcers].

In the pathogenesis of peptic ulcer, development of tissue hypoxia holds a prominent place. Its timely detection and medicamentous correction necessitates development of criteria for its diagnosis. A study was made of activity of the key enzymes of glycolysis and purine metabolism lactatdehydrogenase and adenosinedesaminase in tissues, erythrocytes, and blood plasma in patients with peptic ulcer both in its uncomplicated course and in development of complications.