Publications by authors named "B Uygun"
Article Synopsis
- Uterus transplantation is currently the only solution for women with absolute uterine infertility, such as those with Rokitansky syndrome, allowing them to experience pregnancy and childbirth.
- There are significant challenges with uterus transplantation, including the risks of ischemia-reperfusion injury and a notable thrombotic complication rate of up to 20%, which can lead to graft rejection.
- This protocol aims to outline the surgical steps for obtaining porcine uteruses and implementing dynamic preservation through machine perfusion, which may reduce hypoxic injury compared to traditional static cold storage methods.
Article Synopsis
- * This study introduces a novel technique called subzero nonfreezing (SZNF), where rat hindlimbs are stored at -4°C for 48 hours before transplantation.
- * Results showed that limbs preserved using SZNF survived significantly longer (28 days) compared to those stored using traditional static cold storage (SCS), which failed to survive beyond 4 days, indicating potential for improved VCA preservation methods.
Current methods of storing explanted donor livers at 4 °C in University of Wisconsin (UW) solution result in loss of graft function and ultimately lead to less-than-ideal outcomes post transplantation. Our lab has previously shown that supplementing UW solution with 35-kilodalton polyethylene glycol (PEG) has membrane stabilizing effects for cold stored primary rat hepatocytes in suspension. Expanding on past studies, we here investigate if PEG has the same beneficial effects in an adherent primary rat hepatocyte cold storage model.
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- * A new method called perfusion decellularization creates a scaffold from porcine fasciocutaneous flaps that preserves essential properties such as collagen, microvasculature, and growth factors, making it a promising alternative to traditional flaps.
- * The study found that using 0.2% sodium dodecyl sulfate effectively clears cellular material from the flap while maintaining its structure, indicating biocompatibility as human dermal fibroblasts were able to migrate into the decellularized tissue.
Burn wound healing is a complex and long process. Despite extensive experience, plastic surgeons and specialized teams in burn centers still face significant challenges. Among these challenges, the extent of the burned soft tissue can evolve in the early phase, creating a delicate balance between conservative treatments and necrosing tissue removal.
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