Human pulp-derived cells immortalized with Simian Virus 40 T-antigen.

Primary cells in culture have a limited capacity to divide and soon reach a non-proliferative state. This cellular senescence limits the investigation of cells derived from human pulp concerning cellular pathways, gene regulation, mechanisms of dentin formation, or responses to material exposure. To overcome this problem, primary human pulp-derived cells were established and transfected with a plasmid containing coding sequences of Simian Virus 40 (SV40) large T-antigen.

Clinical evaluation of different adhesive systems for restoring teeth with erosion lesions.

This investigation evaluated the performance of a resin-modified glass ionomer, a compomer, and a bonding system/composite combination for the restoration of cervical erosion lesions without cavity preparation. Forty-eight lesions (11 patients) were restored with a bonding agent/composite combination [Prime & Bond 2.1/PrismaTPH (P & B/TPH); DeTrey/Dentsply], a compomer (Dyract; DeTrey/Dentsply), or a light-curing glass ionomer (Fuji II LC; Fuji).

Partial ceramic crowns: influence of preparation design and luting material on internal adaptation.

The influence of three different cavity preparations on the marginal integrity of partial ceramic crowns (PCC) luted with four different luting systems was investigated in this in vitro study. PCC preparations were performed in 144 extracted human molars using one of the following preparation designs (n=48/preparation): A--Coverage of functional cusps/butt joint preparation; B--horizontal reduction of functional cusps and C--complete reduction of functional cusps/butt joint preparation. Non-functional cusps were not covered; mesial and distal proximal boxes were extended 1 mm below the cemento-enamel-junction.

Partial ceramic crowns. Influence of preparation design and luting material on margin integrity--a scanning electron microscopic study.

This in vitro study examines the effects of three preparation designs and different luting agents on the marginal integrity of partial ceramic crowns. One hundred forty-four extracted human molars were prepared according to the following preparation designs: A. Coverage of functional cusps, B.

Microleakage in class II composite resin restorations: application of a clearing protocol.

Microleakage at the cervical tooth/restoration interface of class II restorations was evaluated, either on multiple sections or on teeth rendered transparent, in order to determine whether a modified clearing protocol could be established as a routine method for microleakage evaluation in class II restorations. Forty-eight class II cavities were restored with a composite/bonding agent (Tetric Universal/Heliobond, Vivadent, Schaan, Liechtenstein) in bulk technique (BT) (n=24) and with a composite/dentin adhesive system (Tetric Universal/Heliobond/Syntac Classic) in increments (IT) (n=24). The 24 specimens were assigned to two groups.