Publications by authors named "B Stamatopoulos"
Extracellular vesicles from chronic lymphocytic leukemia cells promote leukemia aggressiveness by inducing the differentiation of monocytes into nurse-like cells via an RNA-dependent mechanism.
Hemasphere
January 2025
Chronic lymphocytic leukemia (CLL) cells receive several stimuli from surrounding cells, such as B-cell receptor (BCR) stimulation, and can manipulate their microenvironment via extracellular vesicle (EV) release. Here, we investigated the small RNA content (microRNA and YRNA) of CLL-EVs from leukemic cells cultured with/without BCR stimulation. We highlight an increase of miR-155-5p, miR-146a-5p, and miR-132-3p in EVs and in cells after BCR stimulation ( < 0.
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- Mesenchymal stromal cells (MSC) are important for tissue regeneration and immune regulation, but aging affects their function, leading to "inflammaging" and increased disease risk in the elderly, including cancer.
- The study aimed to analyze differences in morphology, gene expression, and function between MSC from young and old healthy donors by using various inflammatory markers and techniques.
- Results showed that aging MSC exhibited changes such as increased size and oxidative stress levels, impaired functionality, and altered gene expression, particularly in their ability to modulate immune responses.
Motivation: The fast and accurate detection of similar geometrical arrangements of protein residues, known as 3D structural motifs, is highly relevant for many applications such as binding region and catalytic site detection, drug discovery and structure conservation analyses. With the recent publication of new protein structure prediction methods, the number of available protein structures is exploding, which makes efficient and easy-to-use tools for identifying 3D structural motifs essential.
Results: We present an open-source Python package that enables the search for both exact and mutated motifs with position-specific residue substitutions.
Article Synopsis
- Extracellular vesicles (EVs), discovered in the late 20th century, play a crucial role in cancer development, particularly in blood-related cancers due to their ability to facilitate communication between cells.
- These vesicles include small EVs (exosomes) and large EVs (microparticles), which exchange proteins, DNA, and RNA, impacting tumor growth and the surrounding microenvironment.
- Current research is focused on their effects and potential treatments for hematological malignancies, with promising advances in understanding their role in disease detection and management.
Subset #201 is a clinically indolent subgroup of patients with chronic lymphocytic leukemia defined by the expression of stereotyped, mutated IGHV4-34/IGLV1-44 BCR Ig. Subset #201 is characterized by recurrent somatic hypermutations (SHMs) that frequently lead to the creation and/or disruption of N-glycosylation sites within the Ig H and L chain variable domains. To understand the relevance of this observation, using next-generation sequencing, we studied how SHM shapes the subclonal architecture of the BCR Ig repertoire in subset #201, particularly focusing on changes in N-glycosylation sites.
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