Development of a probe-based blotting technique for the detection of tobacco streak virus.

Digoxigenin (DIG)-labeled DNA probe was developed for a sensitive and rapid detection of the tobacco streak virus (TSV) isolates in India by dot-blot and tissue print hybridization techniques. DIG-labeled DNA probe complementary to the coat protein (CP) region of TSV sunflower isolate was designed and used to detect the TSV presence at field levels. Dot-blot hybridization was used to check a large number of TSV isolates with a single probe.

Association of a potyvirus with mosaic disease of gherkin (Cucumis anguria L.) in India.

A virus associated with severe mosaic disease of gherkin (Cucumis anguria L.) in south India was identified. The infected plants showed mosaic, vein banding, blistering on malformed leaves and fruits.