Optimized protocol for whole organ decellularization.

Background: The idea of tissue decellularization to gain matrices for tissue engineering is promising. The aim of the present study is to establish a safe and reproducible protocol for solid tissue decellularization that prevents the architecture of the matrix with the inherent vascular network.

Methods: The study was performed in rat kidneys which were decellularized by a SDS-based perfusion protocol.

A novel tool for dynamic cell adhesion studies--the De-Adhesion Number Investigator DANI.

For an optimal implementation of materials, such as, e.g. medical implants in living environments, a thorough characterization of cell adhesion, kinetics and strength is required, as well as a prerequisite e.

Decellularized kidney matrix for perfused bone engineering.

The vascularization of tissue-engineered constructs is yet an unsolved problem. Here, recent work on the decellularization of whole organs has opened new perspectives on tissue engineering. However, existing decellularization protocols last several days and derived biomatrices have only been reseeded with cells from the same tissue origin or stem cells differentiating into these types of tissue.

Antimicrobial peptides and proinflammatory cytokines in periprosthetic joint infection.

Background: Differentiation between septic and aseptic loosening of joint replacements is essential for successful revision surgery, but reliable markers for the diagnosis of low-grade infection are lacking. The present study was performed to assess intra-articular and systemic levels of antimicrobial peptides and proinflammatory cytokines as diagnostic markers for periprosthetic joint infection.

Methods: Fifteen consecutive patients with staphylococcal periprosthetic joint infections and twenty control patients with aseptic loosening of total hip and knee replacements were included in this prospective, single-center, controlled clinical trial.

Expression of the tumor markers sialyl Lewis A, sialyl Lewis X, Lewis Y, Thomsen-Friedenreich antigen, galectin-1 and galectin-3 in human osteoblasts in vitro.

Aim: Lewis antigens and the Thomsen-Friedenreich (TF) antigen are complex glycan structures that modulate processes such as cell adhesion and proliferation and tumor metastasis. The aim of our study was to analyze the expression of sialyl Lewis A (sLeA), sialyl Lewis X (sLeX), Lewis Y (LeY), TF, galectin-1 (Gal-1) and galectin-3 (Gal-3) in human osteoblasts in vitro.

Materials And Methods: The expression of the tumor markers sLeA, sLeX, LeY, TF, Gal-1 and Gal-3 was studied by means of immunohistochemistry on cells grown on chamber slides (2D) and on paraffin sections three-dimensional scaffold-free cultures (3D).