Publications by authors named "B R Blakley"
Article Synopsis
- Ergot alkaloids have harmful effects on livestock, prompting a study on their impact on pregnant sheep's blood vessels and the role of -1 adrenergic receptors.
- Twelve pregnant sheep were divided into control and exposure groups, with the exposure group receiving ergot-contaminated feed for 45 days.
- Results showed significant increases in contractile response in the umbilical artery and vein of the exposed group, leading to lower fetal weights, indicating that current ergot safety limits may need reassessment.
Drug enantiomers can possess vastly different pharmacological properties, yet they are identical in their chemical composition and structural connectivity. Thus, resolving enantiomers poses a great challenge in the field of separation science. Enantiomer separations necessitate interaction of the analyte with a chiral environment─in mass spectrometry-based analysis, a common approach is through a three-point interaction with a chiral selector commonly introduced during sample preparation.
View Article and Find Full Text PDFStructural mass spectrometry (MS) techniques are fast and sensitive analytical methods to identify noncovalent guest/host complexation phenomena for desirable solution-phase properties. Current MS-based studies on guest/host complexes of drug and drug-like molecules are sparse, and there is limited guidance on how to interpret MS information in the context of host nanoencapsulation and inclusion. Here, we use structural MS strategies, combining energy-resolved MS (ERMS), ion mobility-MS (IM-MS), and computational modeling, to characterize 14 chemically distinct drug and drug-like compounds for their propensity to form guest/host complexes with the widely used excipient, beta-cyclodextrin (βCD).
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- The study examined the clinical features and outcomes of 349 dogs in Saskatchewan diagnosed with anticoagulant rodenticide (AR) intoxication between 1999 and 2022.
- Most cases occurred in the warmer months (July-October), with a majority of dogs coming from urban areas and primarily ingesting second-generation rodenticides, particularly bromadiolone.
- Clinical signs such as lethargy, dyspnea, and hemorrhage were noted, and dogs that received blood products showed significantly better survival rates, with 86% of those with AR-induced hemorrhage surviving to discharge.
This study evaluated the effect of feeding ergot contaminated grain continuously or intermittently through backgrounding (BG) and finishing (FN) in a mash or pelleted supplement on the growth performance, health and welfare parameters, and carcass characteristics of feedlot beef steers. Sixty black Angus steers (300 ± 29.4 kg BW) were used in a complete randomized 238-d study.
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