The bacteriophage lambda gt10 DNA containing an insertion of 20 pairs of GC-bases capable of being arranged as Z-form was cloned. Two independent methodological approaches based on the main properties of Z-form were used to study the shape of the insertion: formation of transition bridges composed of unpaired nucleotides between left-rotating Z-forms and right-rotating B-forms of helix (j-domain) and high immunogenic activity of Z-form. O-beta-diethylaminoethylhydroxyamine (OHA), an analogue of hydroxylamine, is capable of reacting specifically with unpaired cytidines.
View Article and Find Full Text PDFThe site of the transition regions between the B- and Z-forms of the DNA duplex (B-Z junction) may serve as marker of the existence of Z-DNA in situ. The structure of (dC-dG)10 insert in the bacteriophage lambda gt10 has been studied in situ by modification of B-Z junction with O-beta-diethylaminoethylhydroxylamine (OHA). The latter is an analogue of hydroxylamine possessing specificity with respect to unpaired cytidine.
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