Dietary fibre counters the oncogenic potential of colibactin-producing Escherichia coli in colorectal cancer.

Diet, microbiome, inflammation and host genetics have been linked to colorectal cancer development; however, it is not clear whether and how these factors interact to promote carcinogenesis. Here we used Il10 mice colonized with bacteria previously associated with colorectal cancer: enterotoxigenic Bacteroides fragilis, Helicobacter hepaticus or colibactin-producing (polyketide synthase-positive (pks)) Escherichia coli and fed either a low-carbohydrate (LC) diet deficient in soluble fibre, a high-fat and high-sugar diet, or a normal chow diet. Colonic polyposis was increased in mice colonized with pks E.

Unlocking the Synergistic Potential and Efficacy of Biosurfactant-Silver Nanoparticle for Enhanced Antimicrobial Activities.

Microorganisms can produce various amphiphilic compounds known as biosurfactants, with diverse applications in distinct industries. This study was focused on the biosurfactant production by Limosilactobacillus fermentum HBUAS62516 for the synthesis of silver nanoparticles. The biosurfactant obtained was characterized as glycolipid using FTIR which showed prominent peaks at 2932.

Mechanism for local attenuation of DNA replication at double-strand breaks.

DNA double-strand breaks (DSBs) disrupt the continuity of the genome, with consequences for malignant transformation. Massive DNA damage can elicit a cellular checkpoint response that prevents cell proliferation. However, how highly aggressive cancer cells, which can tolerate widespread DNA damage, respond to DSBs alongside continuous chromosome duplication is unknown.

Blood biomarker fingerprints in a cohort of patients with CHRNE-related congenital myasthenic syndrome.

Mutations in CHRNE encoding the epsilon subunit of acetylcholine receptor result in impaired neuromuscular transmission and congenital myasthenic syndrome (CMS) with variying severity of symptoms. Although the pathophysiology is well-known, blood biomarker signatures enabling a patient-stratification are lacking. This retrospective two-center-study includes 19 recessive CHRNE-patients (AChR deficiency; mean age 14.

Harnessing tomato-derived small extracellular vesicles as drug delivery system for cancer therapy.

Aim: This study aims to explore a sustainable and scalable approach using tomato fruit-derived sEVs (TsEVs) to deliver calcitriol for enhanced anticancer effects, addressing challenges of low yield and high costs associated with mammalian cell-derived sEVs.

Methods: TsEVs were isolated by centrifugation and ultrafiltration and characterized using DLS, TEM, and biochemical assays. Calcitriol was loaded into TsEVs via loading methods, with efficiency measured by spectrophotometry and HPLC.