Atlas-scale single-cell DNA methylation profiling with sciMETv3.

Single-cell methods to assess DNA methylation have not achieved the same level of cell throughput per experiment compared to other modalities, with large-scale datasets requiring extensive automation, time, and other resources. Here, we describe sciMETv3, a combinatorial indexing-based technique that enables atlas-scale libraries to be produced in a single experiment. To reduce the sequencing burden, we demonstrate the compatibility of sciMETv3 with capture techniques to enrich regulatory regions, as well as the ability to leverage enzymatic conversion, which can yield higher library diversity.

A 4-year outbreak of MRSA ST72-MRSA-IV type t1597 in a surgical high dependency unit in Ireland linked to repeated healthcare worker recolonisation.

Background: Patients undergoing cardiac surgery are identified as high risk for infection, including MRSA. An outbreak of MRSA was identified when two patients experienced MRSA infection concurrently in a cardiothoracic high dependency unit with uncommon detection of MRSA previously and an established screening programme.

Methods: An outbreak control team was convened and interventions applied including refresher training in hand and environmental hygiene, review of practice with regard to aseptic access of medical devices and consideration of antibiotic use in the unit.

Thermophysical Behavior of Carbonated Aqueous Solutions Containing Monoethanolamine and Degradation Products.

The impact of the degradation of monoethanolamine (MEA) on the physicochemical properties of the solvent is experimentally characterized. Based on the identification of three main degradation products of MEA: oxazolidine-2-one (OZD), N-(2-hydroxyethyl)ethylenediamine (HEEDA), and 1-(2-hydroxyethyl)-2-imidazolidinone (HEIA), new measurements for the density, surface tension, and viscosity of partially carbonated solutions containing water, MEA and those products were conducted at different MEA/degradation product molar ratios. The experiments covered a temperature range from 298.

Successful control of an outbreak of Panton-Valentine leucocidin positive meticillin resistant in a National Burns Unit through early detection by whole genome sequencing.

We report an outbreak of PVL-producing MRSA in the Irish National Burns Unit in 2022 involving seven patients, two staff members and two positive environmental samples. This outbreak was successfully controlled using a range of measures including staff screening, environmental screening and enhanced cleaning. The use of real time whole genome sequencing (WGS) allowed for rapid identification of relatedness and for a rapid outbreak response.