Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology.

The application of the xylose reductase (XR) enzyme in the development of biotechnology demands an efficient and large scale enzyme separation technique. The aim of this present work was to optimize xylose reductase (XR) purification process through ultrafiltration membrane (UF) technology using Central composite design (CCD) of response surface methods (RSM). The three effective parameters analyzed were filtration time (0-100), transmembrane pressure (TMP) (1-1.