[Obtaining liposomal preparations of various biologically active substances by the method of detergent flow analysis].

It was shown that detergent dialysis could be successfully used for liposomal encapsulation of substances belonging to different chemical groups with diverse therapeutic activity such as rifampicin, aclarubicin, amphotericin B, pefloxacin and insulin. Liposome encapsulation of substances poorly soluble or insoluble in aqueous media was likely the most promising. The optimal incorporation depended on both the composition of the lipids forming the liposomes and the properties of the compounds being encapsulated.

[Biochemical mechanisms of compensation for unilateral motor disorders of central genesis and their connection with the immune system].

Unilateral impairments of brain led to appearance of posture asymmetry factors (PAF) in central nervous system, which caused asymmetric function of spinal centers and were responsible for asymmetry of limb muscular tension. During compensation symmetrization of muscular tension was followed by inactivation of PAF using developed in central nervous system antifactors which formed complexes with PAF. These factors of inactivation proved to be thermolabile proteins partially involving IgG.

A polyfunctional molecule produced by the conjugation of a synthetic polyion-immunostimulant with specific antigen and an inhibitor of mast cell activation: effects on histamine release.

Polyfunctional molecules were prepared by appropriate conjugation of three moities (1) a polyion-immunostimulant (copolymer of N-vinylpyrrolidone with vinylamine-VP/VA), (2) an inhibitor of mediator secretion from mast cells (diphenylquinone-DPQ) and (3) a specific allergen (ovalbumin-OV). The conjugate of VP/VA with DPQ dose-dependently inhibited histamine release from rat and mouse mast cells induced by the selective releasing agents compound 48/80, ionophore A23187 and specific allergen. The DPQ bound to the polyion was responsible for the inhibitory action of the conjugate.

[Immunogenic activity of synthetic brucellosis antigens].

Experimental data obtained in this investigation indicate that the conjugation of Brucella protective antigen with a polymer carrier essentially increase the immunogenic properties of the antigen. Synthesized vaccinal preparations can be of interest for practical use, as these preparations, while inducing the development of intense immunity, do not impede the diagnosis of brucellosis by serological reactions. The comparative study of the conjugates of Brucella antigen with different carriers shows that the conjugated preparation obtained on the basis of modified dextran possesses high protective potency, which makes it possible to regard this carrier as very promising for further use.