Rutin ameliorates free radical mediated cataract by enhancing the chaperone activity of α-crystallin.

Background: Cataract, the leading cause of blindness, is associated with oxidative damage and protein modification in the lens. The present study was carried out to assess the efficacy of rutin on rat-lens crystallins in selenite-induced in-vivo cataract models.

Methods: Eight-day-old Sprague-Dawley rat pups were grouped as control (G I), experimental (G II) and rutin-treated (G III).

Prevention of selenite induced oxidative stress and cataractogenesis by luteolin isolated from Vitex negundo.

Free radical mediated oxidative stress plays a crucial role in the pathogenesis of cataract and the present study was to determine the efficacy of luteolin in preventing selenite induced oxidative stress and cataractogenesis in vitro. Luteolin is a bioactive flavonoid, isolated and characterized from the leaves of Vitex negundo. Lenses were extracted from Sprague-Dawley strain rats and were organ cultured in DMEM medium.

Analysis on the alterations of lens proteins by Vitex negundo in selenite cataract models.

Purpose: Cataract is the leading cause of blindness and is associated with oxidative damage and protein modification in the lens. In the present study, we have employed proteomic and microscopic approaches to investigate the attenuation of selenite cataract by the flavonoids from Vitex negundo (FVN).

Methods: To demonstrate this attenuation, Sprague-Dawley rat pups were divided into control (G I), selenite induced (G II), and selenite + FVN treated (G III).

Amelioration of selenite toxicity and cataractogenesis in cultured rat lenses by Vitex negundo.

Purpose: Recent evidence suggests that oxidative stress plays a significant role in the development of cataract. The present study sought to evaluate the efficacy of flavonoid fraction of Vitex negundo (FVN) in preventing the toxicity induced by sodium selenite in vitro culture condition.

Methods: Enucleated rat lenses were maintained in organ culture containing DMEM medium alone (G I), supplemented with 0.

Moringa oleifera prevents selenite-induced cataractogenesis in rat pups.

Purpose: The aim of this study was to investigate the protective effects of the flavonoid fraction of Moringa oleifera leaves (FMO) on selenite cataract in vivo.

Methods: Rat pups of Sprague-Dawley strain initially weighing 10–12 g on day 8 were used for the study and grouped as control (G I), selenite induced (G II), and FMO treated (G III). The rat pups in G II and G III received a single subcutaneous injection of sodium selenite (4 µg/g body weight) on day 10 and G III was administered with FMO (2.