[Isolation and functional-structural characteristics of Bacillus polymyxa RP4::Mucts62 transcipients].

Conjugative-like transfer of hybrid plasmid RP4::Mucts62 from Escherichia coli to plasmid-free Bacillus polymyxa was carried out. Bacillus transcipients are detected by the markers of kanamycin and tetracyclin resistance RP4 and thermal sensitivity to growth at 40-42 degrees C, determined by prophage Mucts62 in the plasmid. The technique of transception using millipore filters on solid media has been improved.

[Mu-induced deletions and mutations of Erwinia carotovora chromosomes, including resident prophages E105 and 59].

The plasmid RP4::Mu cts62 in stably inherited by Erwinia carotovora 268 strain. Under the conditions of thermoinduction bacteriophage Mu is segregated and completely eliminated more intensively than in Escherichia coli cells. At thermoinduction the transposition of bacteriophage Mu cts62 into different chromosomal sites takes place, causing the induction of chlorate resistant and auxotrophic mutants with the frequency of 10(-4).

[Glycine-dependent cryotransformation of Bacillus anthracis by DNA of various plasmids].

The possibility of the 1,8-60,0 Md plasmids DNA transfer into the cells of different Bacillus anthracis strains has been established. A number of parameters of the procedure has been optimized. The including of PEG6000 into the cryomixture in the final concentration 10% has been demonstrated to be vital for increase in transformants yields.

[Erwinia carotovora as a recipient system for the natural hybrid plasmid RP4::Mu cts62].

The plasmid RP4::Mu cts62 is transferred from Escherichia coli cells into a recipient strain Erwinia carotovora 268 by conjugation with the frequency 1.5-5 x 10(-7) per donor cell. The maximal frequencies of transfer are obtained by cultivation of donor and recipient cells for 3-5 h on the filters.

[The multiple effect of plasmid RP4::Mu cts 62 in transcipient bacilli].

Transfer of conjugative hybrid plasmid RP4::Mu cts 62 from Escherichia coli into Bac. cereus, Bac. thuringiensis, Bac.