Monkey kidney cell growth optimization and poliovirus propagation in microcarrier culture.

Three monkey kidney cells lines Vero, LLC-MK2 and CV-1 were grown in microcarrier cultures. The carrier support was DEAE-Sephadex gel beads at low anion exchange capacity prepared according to a protocol developed at the Massachusetts Institute of Technology. The growth rate of the cells and the final cell density in microcarrier culture was dependent on the concentration of the beads in culture and on the size of the initial cell inoculum.

Cell growth optimization in microcarrier culture.

Three monkey kidney cell lines and primary chicken embryo cells were grown in microcarrier culture. The carrier support was DEAE-Sephandex gel beads at low anion exchange capacity prepared according to a protocol developed at the Massachusetts Institute of Technology. The growth rate of the cells and the final cell density in microcarrier culture was dependent on the concentration of the beads in culture and on the size of the initial cell inoculum.

[Problems of variability of Mueller-Hinton medium for sensitivity testing. Some parameters and an attempt to standardize sensitivity of Salmonella typhi].

The incidence of some so-called "Mueller-Hinton Medium" for sensitivity testing on the growth of bacteria (especially S. typhi) and on the drug activity are evaluated here. The role of the quality and amount of ingredients : peptone, agar and starch are emphasized.