Intrinsic uncoupling in proton-pumping cytochrome c oxidase: pH dependence of cytochrome c oxidation in coupled and uncoupled phospholipid vesicles.

The pH dependence of the transient aerobic kinetics of cytochromes c and a has been investigated with cytochrome oxidase reconstituted in phospholipid vesicles in the absence and presence of an uncoupler and an ionophore. The cytochrome a reduction level immediately after the burst phase was 60-80% and was not significantly changed by the addition of uncoupler and/or ionophore. The coupled rate of ferro-cytochrome c oxidation increases linearly with decreasing pH in the range 8.

Acid-induced double hits in glutaraldehyde-treated Chlorella.

Glutaraldehyde-treated Chlorella cells show pH-dependent oxygen evolution characteristics. 1. The oxygen yield on the second flash (Y2) of a sequence of 2 mus flashes is pH-dependent, the rest of the sequence is unchanged.

Effects of sodium azide on photosystem II of Chlorella pyrenoidosa.

The action of sodium azide on the electron transport chain was investigated by means of oxygen evolution, fluorescence and luminescence measurements. (1) The damping of the oxygen oscillations is progressively reduced with increasing azide concentration in the range of 10(-5) - 10(-1) M. (2) The rate of the dark decay of the S2 and S3 states is considerably slowed.