Publications by authors named "B M Lobb"
RNA sequencing (RNA-seq) analysis of virus-infected host cells enables researchers to study a wide range of phenomena involving host-virus interactions. This includes genomic analysis of the viral population itself, as well as analysis of the transcriptional dynamics of the virus and host during infection. In this chapter, we provide a guide for researchers interested in performing RNA-seq data analysis of virus-infected host cells or cell lines.
View Article and Find Full Text PDFThe analysis and comparison of gene neighborhoods is a powerful approach for exploring microbial genome structure, function, and evolution. Although numerous tools exist for genome visualization and comparison, genome exploration across large genomic databases or user-generated datasets remains a challenge. Here, we introduce AnnoView, a web server designed for interactive exploration of gene neighborhoods across the bacterial and archaeal tree of life.
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- Acute sinusitis (AS) is a common reason for antibiotic prescriptions in children, making it important to differentiate between bacterial and viral infections to avoid unnecessary antibiotic use.
- This study utilized untargeted RNA sequencing to analyze nasopharyngeal samples from 221 children with diagnosed AS, showing high sensitivity and specificity for detecting pathogens compared to traditional methods like culture and qRT-PCR.
- The findings revealed additional pathogens and distinct host-response signatures that could improve diagnosis and treatment, highlighting the promise of metatranscriptomics in understanding AS more comprehensively.
Article Synopsis
- White-tailed deer (WTD) were studied for SARS-CoV-2 infection in a sample of 258 collected from Québec, Canada, revealing the presence of Delta variant RNA.
- Viral genomic analysis showed clusters of infected deer sequences matched human cases from Vermont, indicating potential cross-species transmission.
- The study identified differences in immune response between humans and infected WTD, highlighting unique host responses to the virus and providing important information for disease surveillance in wildlife.