Morphofunctional analysis of the effects of total sleep deprivation on the CNS in rats.

The aim of the present work was to perform a morphofunctional analysis of the effects of total sleep deprivation of different durations on neuron and synapse ultrastructure in the somnogenic formations (layers III-V of the anterior limbic cortex, field CA1 of the dorsal hippocampus, the pontine reticular formation, the dorsal cervical nucleus, and the locus ceruleus) in rats, with assessment of study investigative, comfort, and sexual behavior, as well as food and water consumption. Reparative changes in neurons and synapses after total sleep deprivation lasting 12-24 h were accompanied by increases in all types of behavioral reactions. Repair processes in neurons and synapses were somewhat weakened after total sleep deprivation lasting 36 h, while dystrophic changes affected greater proportions of neurons and synapses, and the numbers of behavioral reactions decreased.

[Morpho-functional analysis of the effect of total sleep deprivation on CNS].

The aim of this work was to carry out the morpho-functional analysis of the effect of total sleep deprivation (TSD) of different duration on the ultrastructure of the neurons and the synapses of brain somnogenic structures (III--V layers of frontal limbic cortex, CAI field of dorsal hippocampus, reticular formation of pons Varolii, dorsal nucleus raphe and locus coeruleus) in rats as well on exploratory, comfort and sexual behavior and on food and water consumption. It was revealed that in early terms (12-24 hours) of the TSD the changes of reparative disposition were accompanied by intensification of behavioral reactions in the neurons and synapses. Under 36-hours TSD reparative processes are rather reduced in the neurons and synapses, while dystrophic changes covered a large number of neurons and synapses, which are accompanied with decrease of the number of the behavioral reactions.

[The ultrastructural reorganization of the neurons in some brain formations during the deprivation of paradoxical sleep].

Electron microscopy was used to study intracellular changes in the dorsal hippocampus, lateral hypothalamic nucleus and pontine reticular formation of rats after 96-hour paradoxical sleep deprivation. It was found that compensative-accommodative processes predominate in the majority of neurons. At the same time destructive changes are detected in some cells.

[Intracellular regeneration of neurons in the rat lateral hypothalamic area of the brain after resumption of food intake].

Electron microscopy was used to explore changes in intracellular regeneration processes in neurons of the anterior, medial and posterior parts of the lateral hypothalamus area (LHA) of rats at various time (10, 20, 30, 50 and 70 days) after resumption of food perception. Ultrastructural changes observed during 7 days of food deprivation in intact neurons were of a reversible character. Recovery processes initially appeared and finished earlier in the neurons of medial (day 30) and anterior (day 50) parts of the LHA, in the posterior part of LHA the normalization of the neuronal structure was slower and was over only by the 70th day after the resumption of food reception.