Relationship between different stages of the corpus luteum and the expression of the peroxisome proliferator-activated receptor gamma protein in bovine large lutein cells.

Lutein cells produce progestins that support pregnancy. Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism. Peroxisome proliferator-activated receptors (PPAR) are transcription factors that are central in the regulation of lipid metabolism.

Effects of stress-related signal molecules on cells associated with muscle tissue.

Objective: To elucidate the physiologic background that makes muscle hypertrophy, especially that due to strenuous exercise, often parallel to stress sensitivity and signs of acute phase immune response.

Study Design: We used an animal model: lines of mice with hypertrophied (H) and normally developed (N) hind leg muscles, six in each case. Functional and receptor tests on cells from digested muscle tissue were made and analyzed by microplate cytofluorimetry and flow cytometry.

Prediction of growth with kinetics of cell-free translation systems.

The kinetics of cell-free translation systems containing as main components washed rat liver polysomes and muscle cell sap from a muscle piece of the proband, can be used for predicting body growth rate or muscle tissue growth in cattle and swine. A muscle biopsy from the hindquarters of animals aged about 45 days is the source of the muscle fraction. It introduces into the translation systems the required aminoacylated tRNA and translational initiating and elongating factors containing the muscle cell sap fraction of the proband.