Publications by authors named "B L Raymond"
Immortalised cell lines that mimic their primary cell counterparts are fundamental to research, particularly when large cell numbers are required. Here, we report that immortalisation of bone marrow-derived macrophages (iBMDMs) using the J2 virus resulted in the loss of a protein of interest, MSR1, in WT cells by an unknown mechanism. This led us to perform an in-depth mass spectrometry-based proteomic characterisation of common murine macrophage cell lines (J774A.
View Article and Find Full Text PDFPain management in pregnant and postpartum people with an opioid-use disorder (OUD) requires a balance between risks associated with opioid tolerance, including withdrawal or return to opioid use, considerations around social needs of the maternal-infant dyad, and the provision of adequate pain relief for the birth episode that is often characterized as the worst pain a person will experience in their lifetime. This multidisciplinary consensus statement between the Society for Obstetric Anesthesia and Perinatology (SOAP), Society for Maternal-Fetal Medicine (SMFM), and American Society of Regional Anesthesia and Pain Medicine (ASRA) provides a framework for pain management in obstetric patients with OUD. The purpose of this consensus statement is to provide practical and evidence-based recommendations and is targeted to health care providers in obstetrics and anesthesiology.
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- - The study investigates the role of Asaia bacteria as potential symbionts for Aedes aegypti mosquitoes, focusing on their effects on larval development and symbiotic relationships.
- - Experiments involved comparing mosquito larvae with both intact and aseptic microbiomes to determine the impact of three Asaia species, revealing that while Asaia can alter the gut microbiome, it is a transient presence that mainly affects the abundance of other bacteria.
- - Findings indicated that Asaia bacteria have limited benefits for Aedes aegypti, showing species-specific positive effects on larval development primarily through interaction with other gut bacteria, rather than through direct mutualistic benefits.
Multiplexed imaging technologies have made it possible to interrogate complex tissue microenvironments at sub-cellular resolution within their native spatial context. However, proper quantification of this complexity requires the ability to easily and accurately segment cells into their sub-cellular compartments. Within the supervised learning paradigm, deep learning-based segmentation methods demonstrating human level performance have emerged.
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