Crown-of-Thorns Sea Star Acanthaster cf. solaris Has Tissue-Characteristic Microbiomes with Potential Roles in Health and Reproduction.

Outbreaks of coral-eating crown-of-thorns sea stars (CoTS; species complex) cause substantial coral loss; hence, there is considerable interest in developing prevention and control strategies. We characterized the microbiome of captive CoTS and assessed whether dysbiosis was evident in sea stars during a disease event. Most tissue types had a distinct microbiome.

Aerial application of copper for dothistroma control in New Zealand's planted forests-effect on stream environments.

Limited information is available on the risk to aquatic environments from the aerial application of copper fungicides to treat dothistroma needle blight in managed forests. Cuprous oxide was aerially applied to three catchments of Pinus radiata of varying age classes in the central North Island of New Zealand. Copper was monitored in stream water and sediments prior to and for 1 month after application.

Regulation of endothelial-specific transgene expression by the LacI repressor protein in vivo.

Genetically modified mice have played an important part in elucidating gene function in vivo. However, conclusions from transgenic studies may be compromised by complications arising from the site of transgene integration into the genome and, in inducible systems, the non-innocuous nature of inducer molecules. The aim of the present study was to use the vascular system to validate a technique based on the bacterial lac operon system, in which transgene expression can be repressed and de-repressed by an innocuous lactose analogue, IPTG.

Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae.

The development of efficient probiotic application protocols for use in marine larviculture relies on comprehensive understanding of pathogen-probiont-host interactions. The probiont combination of Pseudoalteromonas sp. PP107 and Vibrio sp.

Polymorphism in endothelial connexin40 enhances sensitivity to intraluminal pressure and increases arterial stiffness.

Objective: To determine whether impairment of endothelial connexin40 (Cx40), an effect that can occur in hypertension and aging, contributes to the arterial dysfunction and stiffening in these conditions.

Approach And Results: A new transgenic mouse strain, expressing a mutant Cx40, (Cx40T202S), specifically in the vascular endothelium, has been developed and characterized. This mutation produces nonfunctional hemichannels, whereas gap junctions containing the mutant are electrically, but not chemically, patent.