Meropenem-resistant Burkholderia pseudomallei: a concerning single case in Australia with no prior meropenem exposure.

We report a case of cutaneous melioidosis in a 54-year-old male with a meropenem-resistant sub-population. He was empirically treated with episodic doxycycline and trimethoprim-sulfamethoxazole; however, the abscess re-accumulated. The patient had no prior exposure to meropenem.

A High-Throughput Droplet-based Method to Facilitate Microbial Conjugation.

Droplet microfluidic platforms have been broadly used to facilitate DNA transfer in mammalian and bacterial hosts via methods such as transformation, transfection, and conjugation, as introduced in our previous work. Herein, we recapitulate our method for conjugal DNA transfer between strains in a droplet for increased conjugation efficiency and throughput of an otherwise laborious protocol. By co-incubating the donor and recipient strains in droplets, our method confines cells into close proximity allowing for increased cell-to-cell interactions.

Climate Change and the Emergence of No-Analog Forest Assemblages in North America.

Future climate change is expected to result in tree species shifting their geographic distributions in ways that could reorganize species into assemblages with no contemporary analog. These projected no-analog forests raise concern as their ecological function could similarly shift, which may challenge established conservation and management efforts. Here, we implement a community-level modelling approach to identify the key climatic and topographic drivers of forest composition in North America, and then use these models to predict the distribution of "disappearing" and "novel" forest assemblages in future climate.