Publications by authors named "B J Wainger"
Distinct modulation of calcium-activated chloride channel TMEM16A by drug-binding sites.
Proc Natl Acad Sci U S A
December 2024
Article Synopsis
- * Using magnolol and honokiol, researchers identified two distinct drug-binding sites on TMEM16A—one in the pore region and another nonpore pocket, each interacting differently with the channel.
- * The study highlights that out of 17 inhibitors tested, a majority acted as pore blockers, revealing the significance of the nonpore pocket and informing future drug development strategies.
Article Synopsis
- The study examines the relationship between the physical traits (morphology) of neuromas and the symptoms they cause, aiming to create a classification system to help understand their clinical implications.
- A total of 45 neuroma specimens from 27 patients were analyzed, revealing that atypical neuromas were linked to higher pain levels and were more common in patients with conditions like diabetes and peripheral vascular disease.
- The proposed classification system shows strong reliability and may help surgeons and researchers improve their understanding of neuroma development and its connections to patient health factors, though further research is needed in this area.
While voltage-gated potassium channels have critical roles in controlling neuronal excitability, they also have non-ion-conducting functions. Kv8.1, encoded by the KCNV1 gene, is a 'silent' ion channel subunit whose biological role is complex since Kv8.
View Article and Find Full Text PDFCannabidiol (CBD), the main nonpsychoactive cannabinoid of cannabis, holds promise for nonaddictive treatment of pain. Although preclinical studies have been encouraging, well-controlled human trials have been largely unsuccessful. To investigate this dichotomy and better understand the actions of CBD, we used high-content calcium imaging with automated liquid handling and observed broad inhibition of neuronal activation by a host of ionotropic and metabotropic receptors, including transient receptor potential (Trp) and purinergic receptors, as well as mediators of intracellular calcium cycling.
View Article and Find Full Text PDFBackground: Beyond neuronal injury, cell death pathways may also contribute to vascular injury after stroke. We examined protein networks linked to major cell death pathways and identified SLC22A17 (solute carrier family 22 member 17) as a novel mediator that regulates endothelial tight junctions after ischemia and inflammatory stress.
Methods: Protein-protein interactions and brain enrichment analyses were performed using STRING, Cytoscape, and a human tissue-specific expression RNA-seq database.