Expression of human argininosuccinate synthetase in murine hematopoietic cells in vivo.

As part of an effort to develop a model system for somatic gene therapy, a human argininosuccinate synthetase (AS) cDNA has been introduced into two gag+ retroviral vectors, and high titer clones were obtained for both constructs. The presence of proviral DNA sequences was detected in individual spleen colonies after infection of primary murine marrow cells with each virus. Mice were reconstituted for long-term survival with marrow infected with one virus, and they demonstrated elevated levels of AS expression in peripheral blood for up to eight weeks posttransplantation.

Intestinal parasites of well-cared-for dogs: an area revisited.

A prior study (1970) of parasites in fecal samples of 846 dogs recovered from an area in the Edgewater and Rogers Park areas on the far North side of Chicago showed 13.7% of the fecal samples to be positive for one or more parasites. A pilot study of 200 fecal samples in the same area in 1979 revealed that 21.

Unusual protozoal infestation of the cervical mucus.

The transient colonization of the female genital tract with an unusual multiflagellated protozoan is reported. The presence of active organisms in large numbers in the mucus of the endocervical canal and their absence from the gastrointestinal tract and other areas indicates true colonization rather than contamination. The colonization resolved spontaneously.