Publications by authors named "B Hym"
Background: The precision of the formulae used to estimate glomerular filtration rate (GFR) decreases when the serum creatinine (SCr) assay is biased compared with the assay used during the development of the formulae.
Methods: For 100 children referred for 51Cr-EDTA clearance (CLEDTA), SCr was measured with a JAFFE (classic Jaffe colorimetric creatinine assay), a compensated Jaffe (COMP), an enzymatic (ENZ) and an HPLC assay. A population pharmacokinetics approach based on a non-linear mixed effects model (NONMEM) was used to model the relationships between the CLEDTA and physiopathological/analytical variables.
Article Synopsis
- The study aimed to evaluate the variability and accuracy of different serum creatinine measurement methods, focusing on how standardized calibration affects this variability.
- 30 human serum samples and 3 reference materials were tested using 17 different creatinine assays, comparing results from manufacturers’ calibration methods to two standardized calibration approaches.
- Although the calibration method influenced variability, none of the standardized procedures significantly reduced overall inter-assay variability, confirming the need for caution in using creatinine assays for estimating kidney function.
[Improving the interlaboratory variation for creatinine serum assay].
Ann Biol Clin (Paris)
August 2004
Purpose: To assess inter-assay variation and accuracy of blood creatinine measurements as well as the effect of the standardization of the calibration procedures on inter-assay variation.
Methods: Inter-assay variation and accuracy were assessed using 30 frozen human sera and 3 certified reference materials, which were analysed by 17 creatinine assays (colorimetric: 12, enzymatic: 4, HPLC: 1). Usual calibration procedure was compared with two common calibration procedures using either a reference material (404.
[Comparison of methods for the determination of blood creatinine].
Ann Biol Clin (Paris)
August 2002
Creatinine is the criterion the most widely used for kidney exploration, either directly or through algorithms. Up to now, it appears that methods of creatinine determination are still very heterogenous. The aim of the present study was to compare the different available methods and to evaluate their impact on the formula of predicted clearance.
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