Stress, coping and the job search process: the experience of people with psychiatric disabilities in supported employment.

Ten people with psychiatric disabilities and their supported employment coordinators responded to a standardized, open-ended interview about the stresses experienced during the job search process and the coping strategies used to deal with these stresses. The stress inherent in the potential for rejection and negative valuation was a major theme. The respondents described both problem based and emotion focused coping strategies.

Selective interactions of concanavalin A at lipid membranes on the surface of an optical fiber.

An optical configuration was developed for sampling fluorescence coupled into an optical fiber from evanescent wave excitation of fluorescent materials at a lipid membrane on a quartz fiber surface. Selective interactions of pyrene-labelled concanavalin A located on a phosphatidyl choline-cholesterol lipid membrane with fluorescein isothiocyanate-labelled dextran in bulk aqueous solution were monitored by the intrinsic fluorescence sensing configuration. Monosialoganglioside, G(M1), was employed as a receptor in a phospholipid membrane on an optical fiber for selective measurement of pyrene-labelled concanavalin A in solution.

Fluorescence wavelength, intensity and lifetime for multidimensional transduction of selective interactions of acetylcholine receptor by lipid membranes.

Concurrent analysis of the fluorescence intensity, at different emission wavelengths, of lipid vesicles containing acetylcholine receptor (AChR) labelled with a nitrobenzoxadiazole (NBD) moiety shows that selective interactions with the agonist carbamylcholine can be detected reproducibly by a self-calibration method with muM detection limits. Concurrent analysis of the fluorescence intensity and lifetime of the new probe 4-dicyanomethylene-1,2,3,4-tetrahydromethylquinoline (DCQ) shows that general alterations of lipid membrane structure induced by temperature variation in the head-group region of lipid vesicles can be determined. A general approach to detection of selective interactions is introduced by observation of fluorescence intensity and lifetime changes of the probe NBD-phosphatidyl ethanolamine dispersed in lipid membranes containing unlabelled AChR.

Chemical transduction with fluorescent lipid membranes using selective interactions of acetylcholine receptor with agonist/antagonist and acetylcholine with substrate.

Alterations in the physical structure of vesicles and monolayers of phospholipids and soybean lecithin were monitored by measurement on the average fluorescence intensity changes from N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L-a-phosphatidyl ethanolamine (NBD-PE) located in the lipid matrices. This probe was intimately dispersed at a concentration of 1-2 mol-% in lipid membranes and had an emission sensitive to local environmental structure. Alterations in the structure of soybean lecithin vesicles were induced by the selective interaction of acetylcholine receptor with the agonist carbamylcholine and the antagonist alpha-bungarotoxin.

Towards a fluorescent chemoreceptive lipid membrane-based optode.

Lipid membranes have been deposited on the surface of glass slides and quartz fibres for the development of optical chemical sensors. The fluorescence properties of probes embedded within ordered lipid matrices were sensitive to physical and electrostatic environmental alterations of the membrane caused by various non-selective processes. This work reports criteria for surface deposition of monolayers of phosphatidylcholine-steroid mixtures and stearic acid by Langmuir-Blodgett techniques.