Publications by authors named "B Halgand"
Inflammation significantly influences cellular communication in the oral environment, impacting tissue repair and regeneration. This study explores the role of small extracellular vesicles (sEVs) derived from lipopolysaccharide (LPS)-treated stem cells from the apical papilla (SCAP) in modulating macrophage polarization and osteoblast differentiation. SCAPs were treated with LPS for 24 h, and sEVs from untreated (SCAP-sEVs) and LPS-treated SCAP (LPS-SCAP-sEVs) were isolated via ultracentrifugation and characterized using transmission electron microscopy, Western blot, and Tunable Resistive Pulse Sensing.
View Article and Find Full Text PDFBioactive dextran-based scaffolds from emulsion templates co-stabilized by poly(lactic-co-glycolic acid) nanocarriers.
Colloids Surf B Biointerfaces
January 2025
Article Synopsis
- Porous polymer scaffolds are explored as temporary implants in regenerative medicine to repair damaged tissues, requiring specific qualities like biocompatibility and controlled porosity.
- The text introduces a method for creating bioactive dextran-based scaffolds using high internal phase emulsions combined with poly(lactic-co-glycolic acid) nanoparticles to enhance stability and mechanical properties.
- The research also demonstrates that loading therapeutic proteins like lysozyme into nanoparticles reduces the release rate, allowing for better control over how and when these proteins are released from the scaffold.
Bioprinting is a booming technology, with numerous applications in tissue engineering and regenerative medicine. However, most biomaterials designed for bioprinting depend on the use of sacrificial baths and/or non-physiological stimuli. Printable biomaterials also often lack tunability in terms of their composition and mechanical properties.
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- Senescent cells accumulate in osteoarthritis (OA) joint tissues due to injury, playing a role in OA development, but therapeutic approaches targeting these cells have been ineffective in human patients.
- This study developed models of chondrocyte senescence using etoposide to induce DNA damage-related senescence and chronic exposure to IL-1β for inflammation-related senescence.
- Etoposide effectively induces markers of cellular senescence, making it a reliable model for researching senescence in chondrocytes and could aid in developing therapies for OA.
Osteoarthritis (OA) is an inflammatory joint disease that affects cartilage, subchondral bone, and joint tissues. Undifferentiated Mesenchymal Stromal Cells are a promising therapeutic option for OA due to their ability to release anti-inflammatory, immuno-modulatory, and pro-regenerative factors. They can be embedded in hydrogels to prevent their tissue engraftment and subsequent differentiation.
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